ArticleThe EMBO journal1997
The linker region of the ABC-transporter Ste6 mediates ubiquitination and fast turnover of the protein.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Recent Insights into the Roles of PEST-Containing Nuclear Protein.Molecular biotechnology · 2025Review
- Taking a holistic view of PEST-containing nuclear protein (PCNP) in cancer biology.Cancer medicine · 2019Review
- Polymorphism in ABC transporter genes of Dirofilaria immitis.International journal for parasitology. Drugs and drug resistance · 2017Article
- The feline bile salt export pump: a structural and functional comparison with canine and human Bsep/BSEP.BMC veterinary research · 2013Article
- Repeat polymorphisms in the low-complexity regions of Plasmodium falciparum ABC transporters and associations with in vitro antimalarial responses.Antimicrobial agents and chemotherapy · 2013Article
- The polymorphic linker domain of pfmdr1 is associated with resistance-conferring mutations in Plasmodium falciparum populations from East and West Africa.Antimicrobial agents and chemotherapy · 2013Article
- Biogenesis of the Saccharomyces cerevisiae pheromone a-factor, from yeast mating to human disease.Microbiology and molecular biology reviews : MMBR · 2012Review
- MDR1 function is sensitive to the phosphorylation state of myosin regulatory light chain.Biochemical and biophysical research communications · 2010Article
- Initial polarized bud growth by endocytic recycling in the absence of actin cable-dependent vesicle transport in yeast.Molecular biology of the cell · 2010Article
- The ATP-binding cassette transporter ABCA4: structural and functional properties and role in retinal disease.Advances in experimental medicine and biology · 2010Review
- Dosage rescue by UBC4 restores cell wall integrity in Saccharomyces cerevisiae lacking the myosin type II gene MYO1.Yeast (Chichester, England) · 2007Article
- Control of Ste6 recycling by ubiquitination in the early endocytic pathway in yeast.Molecular biology of the cell · 2005Article
- Ubiquitin-mediated targeting of a mutant plasma membrane ATPase, Pma1-7, to the endosomal/vacuolar system in yeast.Molecular biology of the cell · 2004Article
- Sphingoid base synthesis is required for oligomerization and cell surface stability of the yeast plasma membrane ATPase, Pma1.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- A mutant plasma membrane ATPase, Pma1-10, is defective in stability at the yeast cell surface.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Components of a ubiquitin ligase complex specify polyubiquitination and intracellular trafficking of the general amino acid permease.The Journal of cell biology · 2001Article
- Uptake of the ATP-binding cassette (ABC) transporter Ste6 into the yeast vacuole is blocked in the doa4 Mutant.Molecular biology of the cell · 2001Article
- A family of small coiled-coil-forming proteins functioning at the late endosome in yeast.Molecular biology of the cell · 2001Article
- The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways.Molecular biology of the cell · 2000Article
- Monoubiquitin carries a novel internalization signal that is appended to activated receptors.The EMBO journal · 2000Article
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2 authors.
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Abstract
Upon block of endocytosis, the a-factor transporter Ste6 accumulates in a ubiquitinated form at the plasma membrane. Here we show that the linker region, which connects the two homologous halves of Ste6, contains a signal which mediates ubiquitination and fast turnover of Ste6. This signal was also functional in the context of another plasma membrane protein. Deletion of an acidic stretch in the linker region ('A-box') strongly stabilized Ste6. The A-box contains a sequence motif ('DAKTI') which resembles the putative endocytosis signal of the alpha-factor receptor Ste2 ('DAKSS'). Deletion of the DAKTI sequence also stabilized Ste6 but, however, not as strongly as the A-box deletion. There was a correlation between the half-life of the mutants and the degree of ubiquitination: while ubiquitination of the deltaDAKTI mutant was reduced compared with wild-type Ste6, no ubiquitination could be detected for the more stable deltaA-box variant. Loss of ubiquitination seemed to affect Ste6 trafficking. In contrast to wild-type Ste6, which was associated mainly with internal membranes, the ubiquitination-deficient mutants accumulated at the plasma membrane, as demonstrated by immunofluorescence and cell fractionation experiments. These findings suggest that ubiquitination is required for efficient endocytosis of Ste6 from the plasma membrane.
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