ArticleNucleic acids research1997
Transcription and polyadenylation in a short human intergenic region.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Application of the iPLUS non-coding sequence in improving biopharmaceuticals production.Frontiers in bioengineering and biotechnology · 2024Article
- Enzymatically amplified linear dbDNAGene therapy · 2023Article
- Novel upstream and downstream sequence elements contribute to polyadenylation efficiency.RNA biology · 2012Article
- Alternative splicing and polyadenylation contribute to the generation of hERG1 C-terminal isoforms.The Journal of biological chemistry · 2010Article
- Two G-rich regulatory elements located adjacent to and 440 nucleotides downstream of the core poly(A) site of the intronless melanocortin receptor 1 gene are critical for efficient 3' end processing.Molecular and cellular biology · 2007Article
- Review
- A Kaposi's sarcoma virus RNA element that increases the nuclear abundance of intronless transcripts.The EMBO journal · 2005Article
- The relationship between the prothrombin upstream sequence element and the G20210A polymorphism: the influence of a competitive environment for mRNA 3'-end formation.Nucleic acids research · 2005Article
- Efficient use of a 'dead-end' GA 5' splice site in the human fibroblast growth factor receptor genes.The EMBO journal · 2003Article
- Characterization of specific protein-RNA complexes associated with the coupling of polyadenylation and last-intron removal.Molecular and cellular biology · 2002Article
- Mechanism of poly(A) signal transduction to RNA polymerase II in vitro.Molecular and cellular biology · 2001Article
- Functionally significant secondary structure of the simian virus 40 late polyadenylation signal.Molecular and cellular biology · 2000Article
- Recruitment of a basal polyadenylation factor by the upstream sequence element of the human lamin B2 polyadenylation signal.Molecular and cellular biology · 2000Article
- Assembly of the cleavage and polyadenylation apparatus requires about 10 seconds in vivo and is faster for strong than for weak poly(A) sites.Molecular and cellular biology · 1999Article
- Utilization of splicing elements and polyadenylation signal elements in the coupling of polyadenylation and last-intron removal.Molecular and cellular biology · 1999Article
- Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.Microbiology and molecular biology reviews : MMBR · 1999Review
- Polypyrimidine tract-binding protein positively regulates inclusion of an alternative 3'-terminal exon.Molecular and cellular biology · 1999Article
- SL1 trans splicing and 3'-end formation in a novel class of Caenorhabditis elegans operon.Molecular and cellular biology · 1999Article
- The upstream sequence element of the C2 complement poly(A) signal activates mRNA 3' end formation by two distinct mechanisms.Genes & development · 1998Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The poly(A) signal of the human Lamin B2 gene was previously shown to lie 600 bp upstream of the cap site of a gene of unknown function (ppv 1). However, using RNase protection analysis, we show that ppv 1 has two clusters of multiple initiation sites, so that the 5"cap site lies only approximately 280 nt downstream of the Lamin B2 poly(A) signal. We analysed nascent transcription across this unusually short intergenic region using nuclear run-on analysis of both the endogenous locus and of transiently transfected hybrid constructs. Surprisingly, transcription of the Lamin B2 gene does not appear to terminate prior to any of the mapped ppv 1 start sites, although pausing of the elongating polymerase complexes is observed downstream of the Lamin B2 poly(A) signal. We suggest that this pausing may be sufficient to protect the downstream gene from transcriptional interference. Finally, we have also investigated the sequences required for efficient recognition of the Lamin B2 poly(A) signal. We show that sequences upstream of the AAUAAA element are required for full activity, which is an unusual feature of mammalian poly(A) signals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.