ArticleNucleic acids research1997
Activation of enhancer elements by the homeobox gene Cdx2 is cell line specific.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 72 citations in OpenAlex.
- Intestine-specific homeobox (ISX) induces intestinal metaplasia and cell proliferation to contribute to gastric carcinogenesis.Journal of gastroenterology · 2016Article
- Extending the functions of the homeotic transcription factor Cdx2 in the digestive system through nontranscriptional activities.World journal of gastroenterology · 2015Review
- Helicobacter pylori-Induced Signaling Pathways Contribute to Intestinal Metaplasia and Gastric Carcinogenesis.BioMed research international · 2015Review
- Saltatory remodeling of Hox chromatin in response to rostrocaudal patterning signals.Nature neuroscience · 2013Article
- Article
- Gastrointestinal differentiation marker Cytokeratin 20 is regulated by homeobox gene CDX1.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The homeobox gene CDX2 is aberrantly expressed in most cases of acute myeloid leukemia and promotes leukemogenesis.The Journal of clinical investigation · 2007Article
- Cdx4 dysregulates Hox gene expression and generates acute myeloid leukemia alone and in cooperation with Meis1a in a murine model.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- An early role for WNT signaling in specifying neural patterns of Cdx and Hox gene expression and motor neuron subtype identity.PLoS biology · 2006Article
- Phosphorylation of the homeotic tumor suppressor Cdx2 mediates its ubiquitin-dependent proteasome degradation.Oncogene · 2005Article
- Cdx protein interaction with Hoxa5 regulatory sequences contributes to Hoxa5 regional expression along the axial skeleton.Molecular and cellular biology · 2005Article
- Cdx1 autoregulation is governed by a novel Cdx1-LEF1 transcription complex.Molecular and cellular biology · 2004Article
- Ectopic expression of the homeobox gene Cdx2 is the transforming event in a mouse model of t(12;13)(p13;q12) acute myeloid leukemia.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Effect of gastrin on differentiation of rat intestinal epithelial cells in vitro.World journal of gastroenterology · 2003Article
- A novel colonic repressor element regulates intestinal gene expression by interacting with Cux/CDP.Molecular and cellular biology · 2002Article
- Interaction between the homeodomain proteins Cdx2 and HNF1alpha mediates expression of the lactase-phlorizin hydrolase gene.The Biochemical journal · 2000Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cdx2 is a caudal-related homeodomain transcription factor that is expressed in complex patterns during mouse development and at high levels in the intestinal epithelium of adult mice. Cdx2 activates transcription of intestinal gene promoters containing specific binding sites. Moreover, Cdx2 has been shown to induce intestinal differentiation in cell lines. In this study, we show that Cdx2 is able to bind to two well defined enhancer elements in the HoxC8 gene. We then demonstrate that Cdx2 is able to activate transcription of heterologous promoters when its DNA binding element is placed in an enhancer context. Furthermore, the ability to activate enhancer elements is cell-line dependent. When the Cdx2 activation domain was linked to the Gal4 DNA binding domain, the chimeric protein was able to activate Gal4 enhancer constructs in an intestinal cell line, but was unable to activate transcription in NIH3T3 cells. These data suggest that there are cell-specific factors that allow the Cdx2 activation domain to function in the activation of enhancer elements. We hypothesize that either a co-activator protein or differential phosphorylation of the activation domain may be the mechanism for intestinal cell line-specific function of Cdx2 and possibly in other tissues in early development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.