ArticleInfection and immunity1997
Ultrastructural analysis of primary human urethral epithelial cell cultures infected with Neisseria gonorrhoeae.
Article in Infection and immunity, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 66 citations in OpenAlex.
- Article
- The Neisseria meningitidis Urethritis Clade (NmUC) Acts as a "Chimeric Pathogen" During Infection of Primary, Human Male, Urethral Epithelial Cells.The Journal of infectious diseases · 2025Article
- Article
- Mechanisms of host manipulation byFrontiers in microbiology · 2023Review
- Tissue Models forFrontiers in cellular and infection microbiology · 2022Review
- Epithelial Haven and Autophagy Breakout in Gonococci Infection.Frontiers in cell and developmental biology · 2020Review
- Role of Gonococcal Neisserial Surface Protein A (NspA) in Serum Resistance and Comparison of Its Factor H Binding Properties with Those of Its Meningococcal Counterpart.Infection and immunity · 2019Article
- Pathogenesis ofFrontiers in immunology · 2018Review
- Desialylation of Neisseria gonorrhoeae Lipooligosaccharide by Cervicovaginal Microbiome Sialidases: The Potential for Enhancing Infectivity in Men.The Journal of infectious diseases · 2016Article
- Is gonococcal disease preventable? The importance of understanding immunity and pathogenesis in vaccine development.Critical reviews in microbiology · 2016Review
- Article
- Neisseria gonorrhoeae pilin glycan contributes to CR3 activation during challenge of primary cervical epithelial cells.Cellular microbiology · 2011Article
- The Pathobiology of Neisseria gonorrhoeae Lower Female Genital Tract Infection.Frontiers in microbiology · 2011Article
- Biofilm Formation by Neisseria gonorrhoeae.Infection and immunity · 2005Article
- Gonococcal porin IB activates NF-kappaB in human urethral epithelium and increases the expression of host antiapoptotic factors.Infection and immunity · 2004Article
- The molecular mechanisms used by Neisseria gonorrhoeae to initiate infection differ between men and women.Clinical microbiology reviews · 2004Review
- Gonococcal phospholipase d modulates the expression and function of complement receptor 3 in primary cervical epithelial cells.Infection and immunity · 2003Article
- Immortalization of human urethral epithelial cells: a model for the study of the pathogenesis of and the inflammatory cytokine response to Neisseria gonorrhoeae infection.Infection and immunity · 2002Article
- Intracellular survival of Neisseria gonorrhoeae in male urethral epithelial cells: importance of a hexaacyl lipid A.Infection and immunity · 2002Article
- Modulation of gonococcal piliation by regulatable transcription of pilE.Journal of bacteriology · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In men with gonococcal urethritis, the urethral epithelial cell is a site of infection. To study the pathogenesis of gonorrhea in this cell type, we have developed a method to culture primary human urethral epithelial cells obtained at the time of urologic surgery. Fluorescent analysis demonstrated that 100% of the cells stained for keratin. Microscopic analyses indicated that these epithelial cells arrayed in a pattern similar to that seen in urethral epithelium. Using immunoelectron and confocal microscopy, we compared the infection process seen in primary cells with events occurring during natural infection of the same cell type in men with gonococcal urethritis. Immunoelectron microscopy studies of cells infected with Neisseria gonorrhoeae 1291 Opa+ P+ showed adherence of organisms to the epithelial cell membrane, pedestal formation with evidence of intimate association between the gonococcal and the epithelial cell membranes, and intracellular gonococci present in vacuoles. Confocal studies of primary urethral epithelial cells showed actin polymerization upon infection. Polyclonal antibodies to the asialoglycoprotein receptor (ASGP-R) demonstrated the presence of this receptor on infected cells in the primary urethral cell culture. In situ hybridization using a fluorescent-labeled probe specific to the ASGP-R mRNA demonstrated this message in uninfected and infected cells. These features were identical to those seen in urethral epithelial cells in exudates from males with gonorrhea. Infection of primary urethral cells in culture mimics events seen in natural infection and will allow detailed molecular analysis of gonococcal pathogenesis in a human epithelial cell which is commonly infected.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.