ArticleInfection and immunity1997
Localization of human intestinal defensin 5 in Paneth cell granules.
Article in Infection and immunity, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03311906 (Clinical and Laboratory Effect of 0.8% Hyaluronic Acid Gel in Conventional Treatment of Moderate to Severe Chronic Periodontitis), which is not on this map. Cited by 71 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Clinical and Laboratory Effect of 0.8% Hyaluronic Acid Gel in Conventional Treatment of Moderate to Severe Chronic Periodontitis
Who cites it
71 citing papers in PubMed, 186 citations in OpenAlex.
- α-Defensins and hsCRP levels in inflammatory response of standard and laparoendoscopic single-site cholecystectomy.Surgical endoscopy · 2012Trial
- Comparative evaluation of human ß defensin-2 in GCF and microbial flora in subgingival plaque among patients treated with conventional orthodontic braces and aligners: a prospective clinical study.Dental press journal of orthodontics · 2026Article
- Functional characterization of novel anti-DEFA5 monoclonal antibody clones 1A8 and 4F5 in inflammatory bowel disease colitis tissues.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2025Article
- Anti-DEFA5 Monoclonal Antibody Clones 1A8 and 4F5 Immunoreactive Bioassay for Diagnosing Inflammatory Bowel Disease.Research square · 2024Article
- Application of enzyme-linked immunosorbent assay to detect antimicrobial peptides in human intestinal lumen.Journal of immunological methods · 2024Article
- Infection and inflammation stimulate expansion of a CD74The EMBO journal · 2023Article
- Antimicrobial Peptide Synergies for Fighting Infectious Diseases.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
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- Human intelectin-2 (ITLN2) is selectively expressed by secretory Paneth cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Review
- Article
- Paneth cell α-defensin misfolding correlates with dysbiosis and ileitis in Crohn's disease model mice.Life science alliance · 2020Article
- The Paneth Cell: The Curator and Defender of the Immature Small Intestine.Frontiers in immunology · 2020Review
- Alarin in different human intestinal epithelial cell types.Histochemistry and cell biology · 2019Article
- "Tissue barriers · 2019Review
- Cytotoxic and biological effects of bulk fill composites on rat cortical neuron cells.Odontology · 2018Article
- Human β-defensin-1: A natural antimicrobial peptide present in amniotic fluid that is increased in spontaneous preterm labor with intra-amniotic infection.American journal of reproductive immunology (New York, N.Y. : 1989) · 2018Article
- Expression of α-Defensins, CD20+ B-lymphocytes, and Intraepithelial CD3+ T-lymphocytes in the Intestinal Mucosa of Patients with Liver Cirrhosis: Emerging Mediators of Intestinal Barrier Function.Digestive diseases and sciences · 2018Article
- Entamoeba histolytica Alters Ileal Paneth Cell Functions in Intact and Muc2 Mucin Deficiency.Infection and immunity · 2018Article
- Paneth Cells during Viral Infection and Pathogenesis.Viruses · 2018Review
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Antibiotic peptides of higher animals include the defensins, first discovered in phagocytic cells but recently also found to be produced by epithelial cells. We biosynthesized recombinant human intestinal defensin 5 (rHD-5) using the baculovirus-insect cell expression system. Since insect cells process defensin incompletely and secrete the precursor proHD-5, we substituted a methionine for an alanine at a likely processing site to allow selective chemical cleavage with cyanogen bromide, and rHD-5 was used to elicit polyclonal antibodies. By the immunoperoxidase-staining technique, the antibodies selectively stained Paneth cells of the normal adult small intestine. Immunogold electron microscopy further localized HD-5 to the Paneth cell secretory granules. Since some defensins exert activity cytotoxic to mammalian cells, we assayed the effect of rHD-5 on the human intestinal cell lines Caco2 and Int407. proHD-5 did not exert cytotoxic activity, and rHD-5 showed only minimal activity against Int407 and was inert against Caco2. Since Paneth cells release their granules adjacent to the mitotic cells of the intestinal crypts, HD could protect this cell population against invasion and parasitization by microbes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.