ArticleInfection and immunity1997
A third secreted protein that is encoded by the enteropathogenic Escherichia coli pathogenicity island is required for transduction of signals and for attaching and effacing activities in host cells.
Article in Infection and immunity, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
57 citing papers in PubMed, 171 citations in OpenAlex.
- The sequence of events of enteropathogeniciScience · 2024Article
- The Assembly of Flagella in EnteropathogenicInternational journal of molecular sciences · 2022Article
- Transmembrane domains of type III-secreted proteins affect bacterial-host interactions in enteropathogenicVirulence · 2021Article
- The Evasive Enemy: Insights into the Virulence and Epidemiology of the Emerging Attaching and Effacing PathogenInfection and immunity · 2019Review
- Type Three Secretion System in Attaching and Effacing Pathogens.Frontiers in cellular and infection microbiology · 2016Review
- Pore-forming Activity of the Escherichia coli Type III Secretion System Protein EspD.The Journal of biological chemistry · 2015Article
- The N-terminal amphipathic region of the Escherichia coli type III secretion system protein EspD is required for membrane insertion and function.Molecular microbiology · 2011Article
- Atypical enteropathogenic Escherichia coli that contains functional locus of enterocyte effacement genes can be attaching-and-effacing negative in cultured epithelial cells.Infection and immunity · 2011Article
- N-terminal type III secretion signal of enteropathogenic Escherichia coli translocator proteins.Journal of bacteriology · 2010Article
- The RNA binding protein CsrA is a pleiotropic regulator of the locus of enterocyte effacement pathogenicity island of enteropathogenic Escherichia coli.Infection and immunity · 2009Article
- Effect of molecules secreted by Lactobacillus acidophilus strain La-5 on Escherichia coli O157:H7 colonization.Applied and environmental microbiology · 2009Article
- Rapid site-directed domain scanning mutagenesis of enteropathogenic Escherichia coli espD.Biological procedures online · 2007Article
- Mechanism underlying inhibition of intestinal apical Cl/OH exchange following infection with enteropathogenic E. coli.The Journal of clinical investigation · 2007Article
- Bundle-forming pili and EspA are involved in biofilm formation by enteropathogenic Escherichia coli.Journal of bacteriology · 2006Article
- Analysis of the function of enteropathogenic Escherichia coli EspB by random mutagenesis.Infection and immunity · 2006Article
- ClpXP protease controls expression of the type III protein secretion system through regulation of RpoS and GrlR levels in enterohemorrhagic Escherichia coli.Journal of bacteriology · 2005Article
- Enteropathogenic and enterohemorrhagic Escherichia coli infections: translocation, translocation, translocation.Infection and immunity · 2005Review
- Mast cells limit systemic bacterial dissemination but not colitis in response to Citrobacter rodentium.Infection and immunity · 2005Article
- Polarity of enteropathogenic Escherichia coli EspA filament assembly and protein secretion.Journal of bacteriology · 2005Article
- The serine protease motif of EspC from enteropathogenic Escherichia coli produces epithelial damage by a mechanism different from that of Pet toxin from enteroaggregative E. coli.Infection and immunity · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Enteropathogenic Escherichia coli strains are able to signal host cells, cause dramatic cytoskeletal rearrangements, and adhere intimately to the cell surface in a process known as the attaching and effacing effect. A pathogenicity island of 35 kb known as the locus of enterocyte effacement (LEE) is necessary and sufficient for this effect. The LEE encodes an outer membrane adhesin called intimin, a type III secretion apparatus, and the EspA and EspB secreted proteins. The DNA sequence of the region between espA and espB revealed a new gene, espD. The product of espD was demonstrated by using a T7 expression system. We constructed a nonpolar mutation in espD and found that the mutant is incapable of the signal transduction events that lead to activation of the putative intimin receptor in host cells and that the mutant fails to induce the attaching and effacing effect. These phenotypes were restored to the mutant by complementation with a plasmid containing the cloned espD locus. We demonstrated by immunoblotting and microsequencing that the EspD protein is secreted via the type III apparatus. Thus, we describe a novel locus encoding a secreted protein that is required for attaching and effacing activity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.