ArticleInfection and immunity1997
The Yersinia enterocolitica GsrA stress protein, involved in intracellular survival, is induced by macrophage phagocytosis.
Article in Infection and immunity, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 30 citations in OpenAlex.
- Characterization of Toxoplasma DegP, a rhoptry serine protease crucial for lethal infection in mice.PloS one · 2017Article
- Environmental Regulation of Yersinia Pathophysiology.Frontiers in cellular and infection microbiology · 2016Review
- Further characterization of a highly attenuated Yersinia pestis CO92 mutant deleted for the genes encoding Braun lipoprotein and plasminogen activator protease in murine alveolar and primary human macrophages.Microbial pathogenesis · 2015Article
- Human and animal isolates of Yersinia enterocolitica show significant serotype-specific colonization and host-specific immune defense properties.Infection and immunity · 2013Article
- Comparative Global Gene Expression Profiles of Wild-Type Yersinia pestis CO92 and Its Braun Lipoprotein Mutant at Flea and Human Body Temperatures.Comparative and functional genomics · 2010Article
- HtrA stress protein is involved in intramacrophagic replication of adherent and invasive Escherichia coli strain LF82 isolated from a patient with Crohn's disease.Infection and immunity · 2005Article
- The Cpx envelope stress response affects expression of the type IV bundle-forming pili of enteropathogenic Escherichia coli.Journal of bacteriology · 2005Article
- Proteome analysis of secreted proteins of the gastric pathogen Helicobacter pylori.Infection and immunity · 2002Article
- The extracytoplasmic sigma factor, final sigma(E), is required for intracellular survival of nontypeable Haemophilus influenzae in J774 macrophages.Infection and immunity · 2002Article
- The ClpXP ATP-dependent protease regulates flagellum synthesis in Salmonella enterica serovar typhimurium.Journal of bacteriology · 2002Article
- Evaluation of Mycobacterium tuberculosis genes involved in resistance to killing by human macrophages.Infection and immunity · 2000Article
- The alternative sigma factor, sigmaE, is critically important for the virulence of Salmonella typhimurium.Infection and immunity · 1999Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Yersinia enterocolitica gsrA gene is a stress protein gene which was originally identified as essential for protecting cells under both extracellular environmental stress and intracellular stress in macrophages due to phagocytosis. The gsrA gene was shown to be a member of the htrA class of genes and to possess a sequence homologous to that of the promoter recognized by a stress-induced sigma factor, sigmaE. In order to study the induction of the potentially sigmaE-controlled gsrA gene in Y. enterocolitica after phagocytosis by macrophages, we identified GsrA by overproducing the protein using a T7 promoter-gsrA fusion. We found that it is translated as an unstable 49,500-Da protein which is processed by removal of an amino acid fragment consisting of 27 residues, resulting in a stable 46,800-Da protein. By radiolabeling proteins specific to bacteria in the J774-1 macrophage-like cell line, we found that the production of GsrA protein is indeed enhanced in bacterial cells growing within macrophage phagosomes. Transcriptional activation of the gsrA gene was determined by using the gsrA promoter-lacZ fusion system. This work provides the first piece of evidence that the sigmaE regulon responds to the stressful environment found in macrophages.
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