ArticleThe Biochemical journal1997
Diacylglycerol generated by exogenous phospholipase C activates the mitogen-activated protein kinase pathway independent of Ras- and phorbol ester-sensitive protein kinase C: dependence on protein kinase C-zeta.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 75 citations in OpenAlex.
- Lipidomic Signature of Progression of Chronic Kidney Disease in the Chronic Renal Insufficiency Cohort.Kidney international reports · 2016Article
- Regulation of neural progenitor cell proliferation by D609: potential role for ERK.Molecular neurobiology · 2013Article
- The role of atypical protein kinase C in CSF-1-dependent Erk activation and proliferation in myeloid progenitors and macrophages.PloS one · 2011Article
- Regulation of connexin43 gap junctional communication by phosphatidylinositol 4,5-bisphosphate.The Journal of cell biology · 2007Article
- Silica induces macrophage cytokines through phosphatidylcholine-specific phospholipase C with hydrogen peroxide.American journal of respiratory cell and molecular biology · 2007Article
- Activation of alpha1A-adrenergic receptor promotes differentiation of rat-1 fibroblasts to a smooth muscle-like phenotype.BMC cell biology · 2004Article
- Protein kinase Czeta regulates phospholipase D activity in rat-1 fibroblasts expressing the alpha1A adrenergic receptor.BMC cell biology · 2004Article
- Mitogenic synergy through multilevel convergence of hepatocyte growth factor and interleukin-4 signaling pathways.Oncogene · 2002Article
- Regulation of phospholipase D isoenzymes by transforming Ras and atypical protein kinase C-iota.The Biochemical journal · 2001Article
- Protein kinase C activation by acidic proteins including 14-3-3.The Biochemical journal · 2000Article
- Protein kinase C zeta isoform is critical for proliferation in human glioblastoma cell lines.Journal of neuro-oncology · 2000Article
- P(2Y) purinoceptor subtypes recruit different mek activators in astrocytes.British journal of pharmacology · 2000Article
- 14-3-3 isotypes facilitate coupling of protein kinase C-zeta to Raf-1: negative regulation by 14-3-3 phosphorylation.The Biochemical journal · 2000Article
- Bradykinin B(2) receptor-mediated mitogen-activated protein kinase activation in COS-7 cells requires dual signaling via both protein kinase C pathway and epidermal growth factor receptor transactivation.Molecular and cellular biology · 1999Article
- Phosphatidylcholine-specific phospholipase C regulates glutamate-induced nerve cell death.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Localization of atypical protein kinase C isoforms into lysosome-targeted endosomes through interaction with p62.Molecular and cellular biology · 1998Article
- Platelet-derived growth factor activation of mitogen-activated protein kinase depends on the sequential activation of phosphatidylcholine-specific phospholipase C, protein kinase C-zeta and Raf-1.The Biochemical journal · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of diacylglycerol (DG) formation from phosphatidylcholine in mitogenic signal transduction is poorly understood. We have generated this lipid at the plasma membrane by treating Rat-1 fibroblasts with bacterial phosphatidylcholine-specific phospholipase C (PC-PLC). This treatment leads to activation of mitogen-activated protein kinase (MAPK). However, unlike platelet-derived growth factor (PDGF) or epidermal growth factor (EGF), PC-PLC fails to activate Ras and to induce DNA synthesis, and activates MAPK only transiently (<45 min). Down-regulation of protein kinase C (PKC) -alpha, -delta and -epsilon isotypes has little or no effect on MAPK activation by either PC-PLC or growth factors. However, Ro 31-8220, a highly selective inhibitor of all PKC isotypes, including atypical PKC-zeta but not Raf-1, blocks MAPK activation by PDGF and PC-PLC, but not that by EGF, suggesting that atypical PKC mediates the PDGF and PC-PLC signal. In line with this, PKC-zeta is activated by PC-PLC and PDGF, but not by EGF, as shown by a kinase assay in vitro, using biotinylated epsilon-peptide as a substrate. Furthermore, dominant-negative PKC-zeta inhibits, while (wild-type) PKC-zeta overexpression enhances MAPK activation by PDGF and PC-PLC. The results suggest that DG generated by PC-PLC can activate the MAPK pathway independent of Ras and phorbol-ester-sensitive PKC but, instead, via PKC-zeta.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.