ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1997
Expression of specific tubulin isotypes increases during regeneration of injured CNS neurons, but not after the application of brain-derived neurotrophic factor (BDNF).
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 81 citations in OpenAlex.
- Generation of a Transplantable Population of Human iPSC-Derived Retinal Ganglion Cells.Frontiers in cell and developmental biology · 2020Article
- Boosting Central Nervous System Axon Regeneration by Circumventing Limitations of Natural Cytokine Signaling.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Article
- MAG, myelin and overcoming growth inhibition in the CNS.Frontiers in molecular neuroscience · 2015Review
- β-III-Tubulin: a reliable marker for retinal ganglion cell labeling in experimental models of glaucoma.International journal of ophthalmology · 2015Article
- Interleukin-6 contributes to CNS axon regeneration upon inflammatory stimulation.Cell death & disease · 2013Article
- Gene therapy for retinal ganglion cell neuroprotection in glaucoma.Gene therapy · 2012Review
- Taxol facilitates axon regeneration in the mature CNS.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Cortical gene expression in spinal cord injury and repair: insight into the functional complexity of the neural regeneration program.Frontiers in molecular neuroscience · 2011Article
- Interneuronal growth and expression of interneuronal markers in visual cortex of mice with transgenic activation of Ras.Experimental brain research · 2009Article
- Genes associated with adult axon regeneration promoted by olfactory ensheathing cells: a new role for matrix metalloproteinase 2.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Actions of neurotrophic factors and their signaling pathways in neuronal survival and axonal regeneration.Molecular neurobiology · 2006Review
- Exercise-induced gene expression changes in the rat spinal cord.Gene expression · 2005Article
- The upregulated expression of sonic hedgehog in motor neurons after rat facial nerve axotomy.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- An element in the alpha1-tubulin promoter is necessary for retinal expression during optic nerve regeneration but not after eye injury in the adult zebrafish.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Neurotrophic factors and receptors in the immature and adult spinal cord after mechanical injury or kainic acid.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Inactivation of Rho signaling pathway promotes CNS axon regeneration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Regeneration and transplantation of the optic nerve: developing a clinical strategy.The British journal of ophthalmology · 1998Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Axonal regrowth after injury is accompanied by changes in the expression of tubulin, but the contributions of substrate molecules and neurotrophic factors in regulating these changes in vivo are not known. Adult rat retinal ganglion cells (RGCs) were examined after intraorbital axotomy, after application of a peripheral nerve (PN) graft to stimulate regeneration, and after axotomy and treatment with brain-derived neurotrophic factor (BDNF). After these treatments we used in situ hybridization to study mRNA levels for betaI, betaII, betaIII, betaIVa, and Talpha1 tubulin isotypes. Levels of mRNA for all isotypes were downregulated after intraorbital axotomy. During regrowth of injured RGC axons, mRNA levels for betaII, betaIII, and Talpha1 isotypes were upregulated specifically and dramatically, suggesting that elevated expression of these isotypes is correlated specifically with axonal regrowth. A corresponding increase in betaIII protein levels was detected by immunocytochemistry. The betaI and betaIVa mRNAs were not increased during regeneration. BDNF did not elicit a specific increase in the mRNA levels for the betaIII and Talpha1 isotypes and had only a small effect on mRNA levels for the betaII isotype. Therefore, despite the ability of BDNF to support the survival of injured RGCs and to enhance neurite outgrowth of retinal neurons in vitro, the in vivo application of BDNF alone is unable to induce the program of changes in growth-associated tubulins that accompany regeneration of RGC axons into PN grafts. We speculate that, in addition to BDNF, cooperative signaling with substrate molecules is required to allow RGCs to regenerate and exhibit tubulin isotype switching.
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Identifiers
9169523PMC6573326W1595677918What OpenQuestion holds
Registered trials
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