ArticleMolecular biology of the cell1997
Role of Dictyostelium racE in cytokinesis: mutational analysis and localization studies by use of green fluorescent protein.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 74 citations in OpenAlex.
- Discovery and Quantitative Dissection of Cytokinesis Mechanisms Using Dictyostelium discoideum.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Review
- Interactions and Cytotoxicity of Human Neurodegeneration- Associated Proteins Tau and α-Synuclein in the Simple ModelFrontiers in cell and developmental biology · 2021Article
- Phosphorylated Rho-GDP directly activates mTORC2 kinase towards AKT through dimerization with Ras-GTP to regulate cell migration.Nature cell biology · 2019Article
- Functional integrity of the contractile actin cortex is safeguarded by multiple Diaphanous-related formins.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Quantitative imaging of Rac1 activity in Dictyostelium cells with a fluorescently labelled GTPase-binding domain from DPAKa kinase.Histochemistry and cell biology · 2016Article
- Coronin7 regulates WASP and SCAR through CRIB mediated interaction with Rac proteins.Scientific reports · 2015Article
- Rho GTPases orient directional sensing in chemotaxis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Cytokinesis through biochemical-mechanical feedback loops.Seminars in cell & developmental biology · 2010Review
- 14-3-3 coordinates microtubules, Rac, and myosin II to control cell mechanics and cytokinesis.Current biology : CB · 2010Article
- Silent information regulator 2 proteins encoded by Cryptosporidium parasites.Parasitology research · 2010Article
- Yersinia outer protein YopE affects the actin cytoskeleton in Dictyostelium discoideum through targeting of multiple Rho family GTPases.BMC microbiology · 2009Article
- RacG regulates morphology, phagocytosis, and chemotaxis.Eukaryotic cell · 2006Article
- Balance of actively generated contractile and resistive forces controls cytokinesis dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The identification of pats1, a novel gene locus required for cytokinesis in Dictyostelium discoideum.Molecular biology of the cell · 2003Article
- Defects in cytokinesis, actin reorganization and the contractile vacuole in cells deficient in RhoGDI.The EMBO journal · 2002Article
- Signal transduction pathways regulated by Rho GTPases in Dictyostelium.Journal of muscle research and cell motility · 2002Review
- Dictyostelium cytokinesis: from molecules to mechanics.Journal of muscle research and cell motility · 2002Review
- The actin-bundling protein cortexillin is the downstream target of a Rac1-signaling pathway required for cytokinesis.Journal of muscle research and cell motility · 2002Article
- Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth.The EMBO journal · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The small GTPase racE is essential for cytokinesis in Dictyostelium but its precise role in cell division is not known. To determine the molecular mechanism of racE function, we undertook a mutational analysis of racE. The exogenous expression of either wild-type racE or a constitutively active V20racE mutant effectively rescues the cytokinesis deficiency of racE null cells. In contrast, a constitutively inactive N25racE mutant fails to rescue the cytokinesis deficiency. Thus, cytokinesis requires only the activation of racE by GTP and not the inactivation of racE by hydrolysis of GTP. To determine the spatial distribution of racE, we created a fusion protein with GFP at the amino terminus of racE. Remarkably, GFP-racE fusion protein was fully competent to rescue the phenotype of racE null cells and, therefore, must reside in the same location as native racE. We found that GFP-racE localized to the plasma membrane of the cell throughout the entire cell cycle. Furthermore, constitutively active and inactive GFP-racE fusion proteins also localized to the plasma membrane. We mapped the domain required for plasma membrane localization to the carboxyl-terminal 40 amino acids of racE. This domain, however, is not sufficient to confer racE function onto a closely related GTPase. Taken together, these results suggest that racE functions at the cell cortex but it is not involved in determining the timing or placement of the contractile ring.
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