ArticleBiophysical journal1997
Multiphoton excitation fluorescence microscopy and spectroscopy of in vivo human skin.
Article in Biophysical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
68 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Imaging and quantifying drug delivery in skin - Part 2: Fluorescence andvibrational spectroscopic imaging methods.Advanced drug delivery reviews · 2020Pooled it
- Five-modal multiphoton microscopy enabled by a tunable dual-wavelength fiber laser.Biomedical optics express · 2026Article
- Advanced Microscopy Techniques.Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer · 2026Review
- Fluorescence Lifetime Imaging Techniques-A Review on Principles, Applications and Clinical Relevance.Journal of biophotonics · 2025Review
- Towards next-generation diagnostic pathology: AI-empowered label-free multiphoton microscopy.Light, science & applications · 2024Review
- Skin pore imaging using spectral-domain optical coherence tomography: a case report.Biomedical engineering letters · 2023Article
- Imaging guidance for cholesteatoma surgery using tissue autofluorescence.Journal of biomedical optics · 2023Article
- Skin cancer margin detection using nanosensitive optical coherence tomography and a comparative study with confocal microscopy.Biomedical optics express · 2022Article
- Image improvement of temporal focusing multiphoton microscopy via superior spatial modulation excitation and Hilbert-Huang transform decomposition.Scientific reports · 2022Article
- Phototoxicity induced in living HeLa cells by focused femtosecond laser pulses: a data-driven approach.Biomedical optics express · 2021Article
- Nicotinamide effects on the metabolism of human fibroblasts and keratinocytes assessed by quantitative, label-free fluorescence imaging.Biomedical optics express · 2021Article
- Automatic segmentation of skin cells in multiphoton data using multi-stage merging.Scientific reports · 2021Article
- Label-Free Multiphoton Microscopy: Much More Than Fancy Images.International journal of molecular sciences · 2021Review
- Label-Free Multimodal Multiphoton Intravital Imaging.Advances in experimental medicine and biology · 2021Article
- Fast, large area multiphoton exoscope (FLAME) for macroscopic imaging with microscopic resolution of human skin.Scientific reports · 2020Article
- Article
- Simultaneous label-free autofluorescence and multi-harmonic imaging revealsBiomedical optics express · 2019Article
- Light sheet microscopy for histopathology applications.Biomedical engineering letters · 2019Review
- Multimodal imaging platform for optical virtual skin biopsy enabled by a fiber-based two-color ultrafast laser source.Biomedical optics express · 2019Article
- Investigation of psoriasis skin tissue by label-free multi-modal imaging: a case study on a phototherapy-treated patient.Psoriasis (Auckland, N.Z.) · 2019Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Multiphoton excitation microscopy at 730 nm and 960 nm was used to image in vivo human skin autofluorescence from the surface to a depth of approximately 200 microm. The emission spectra and fluorescence lifetime images were obtained at selected locations near the surface (0-50 microm) and at deeper depths (100-150 microm) for both excitation wavelengths. Cell borders and cell nuclei were the prominent structures observed. The spectroscopic data suggest that reduced pyridine nucleotides, NAD(P)H, are the primary source of the skin autofluorescence at 730 nm excitation. With 960 nm excitation, a two-photon fluorescence emission at 520 nm indicates the presence of a variable, position-dependent intensity component of flavoprotein. A second fluorescence emission component, which starts at 425 nm, is observed with 960-nm excitation. Such fluorescence emission at wavelengths less than half the excitation wavelength suggests an excitation process involving three or more photons. This conjecture is further confirmed by the observation of the super-quadratic dependence of the fluorescence intensity on the excitation power. Further work is required to spectroscopically identify these emitting species. This study demonstrates the use of multiphoton excitation microscopy for functional imaging of the metabolic states of in vivo human skin cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.