ArticleMammalian genome : official journal of the International Mammalian Genome Society1997
Mapping of 22 expressed sequence tags isolated from a porcine small intestine cDNA library.
Article in Mammalian genome : official journal of the International Mammalian Genome Society, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 41 citations in OpenAlex.
- A robust linkage map of the porcine autosomes based on gene-associated SNPs.BMC genomics · 2009Article
- A physical map of large segments of pig chromosome 7q11-q14: comparative analysis with human chromosome 6p21.Mammalian genome : official journal of the International Mammalian Genome Society · 2004Article
- Comparative mapping in the pig: localization of 214 expressed sequence tags.Mammalian genome : official journal of the International Mammalian Genome Society · 2003Article
- Improvement of the porcine transcription map: localization of 33 genes, of which 24 are orthologous.Mammalian genome : official journal of the International Mammalian Genome Society · 1998Article
- Chromosomal assignments of 19 porcine cDNA sequences by FISH.Mammalian genome : official journal of the International Mammalian Genome Society · 1998Article
- FISH mapping of seven cDNA sequences in the pig.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1997Article
- Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism analysis.Genome research · 1997Article
- The cytogenetic map of the domestic pig.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Complementary DNA sequences were selected from a resource of tentatively identified clones from a porcine small intestine cDNA library. Forty PCR primer pairs were designed to amplify 101-309 base pairs of the 3' untranslated region of the genes. The PCR conditions were optimized by altering both formamide and magnesium concentrations on samples of pig, mouse, and hamster DNA. Twenty primer pairs that, under stringent conditions, were pig-specific and amplified the expected fragments were chosen for regional assignment in a pig/rodent hybrid cell panel. Furthermore, 22 primer pairs were chosen to amplify DNA from the parental animals of the PiGMaP shared reference families in order to detect possible polymorphisms. Primer pairs that generated polymorphisms were used for genetic mapping. A total of 22 porcine expressed sequence tags (ESTs) were cytogenetically or genetically mapped by this approach. Twelve of the mapped ESTs could be added to the human-porcine comparative map.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.