ArticleThe Journal of cell biology1997
Protein kinase C activation upregulates intercellular adhesion of alpha-catenin-negative human colon cancer cell variants via induction of desmosomes.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 96 citations in OpenAlex.
- Two δ-catenins, plakophilin 4 and p120, promote formation of distinct types of adherens junctions.The Journal of cell biology · 2026Article
- Alpha T-catenin: a crucial tumor suppressor in cancer pathogenesis.Journal of molecular histology · 2024Review
- The interaction of bile acids and gut inflammation influences the pathogenesis of inflammatory bowel disease.Internal and emergency medicine · 2023Review
- Appropriate tension sensitivity of α-catenin ensures rounding morphogenesis of epithelial spheroids.Cell structure and function · 2022Article
- Specific substrates composed of collagen and fibronectin support the formation of epithelial cell sheets by MDCK cells lacking α-catenin or classical cadherins.Cell and tissue research · 2021Article
- The desmosome as a model for lipid raft driven membrane domain organization.Biochimica et biophysica acta. Biomembranes · 2020Review
- Tyrosine-Based Signals Regulate the Assembly of Daple⋅PARD3 Complex at Cell-Cell Junctions.iScience · 2020Article
- Force-dependent allostery of the α-catenin actin-binding domain controls adherens junction dynamics and functions.Nature communications · 2018Article
- α-Catenin homodimers are recruited to phosphoinositide-activated membranes to promote adhesion.The Journal of cell biology · 2017Article
- 293 cells express both epithelial as well as mesenchymal cell adhesion molecules.International journal of molecular medicine · 2016Article
- Computational Screening of Tip and Stalk Cell Behavior Proposes a Role for Apelin Signaling in Sprout Progression.PloS one · 2016Article
- Wnt antagonist SFRP1 functions as a secreted mediator of senescence.Molecular and cellular biology · 2012Article
- Vinculin associates with endothelial VE-cadherin junctions to control force-dependent remodeling.The Journal of cell biology · 2012Article
- Desmosome dynamics in migrating epithelial cells requires the actin cytoskeleton.Experimental cell research · 2011Article
- Desmosomes in the testis: Moving into an unchartered territory.Spermatogenesis · 2011Article
- Intercellular junction assembly, dynamics, and homeostasis.Cold Spring Harbor perspectives in biology · 2010Review
- Molecular events associated with epithelial to mesenchymal transition of nasopharyngeal carcinoma cells in the absence of Epstein-Barr virus genome.Journal of biomedical science · 2009Article
- Plakophilins: multifunctional scaffolds for adhesion and signaling.Current opinion in cell biology · 2009Review
- Protein kinase C isoenzymes differentially regulate the differentiation-dependent expression of adhesion molecules in human epidermal keratinocytes.Experimental dermatology · 2009Article
- Plakophilin 2: a critical scaffold for PKC alpha that regulates intercellular junction assembly.The Journal of cell biology · 2008Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The alpha-catenin molecule links E-cadherin/ beta-catenin or E-cadherin/plakoglobin complexes to the actin cytoskeleton. We studied several invasive human colon carcinoma cell lines lacking alpha-catenin. They showed a solitary and rounded morphotype that correlated with increased invasiveness. These round cell variants acquired a more normal epithelial phenotype upon transfection with an alpha-catenin expression plasmid, but also upon treatment with the protein kinase C (PKC) activator 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Video registrations showed that the cells started to establish elaborated intercellular junctions within 30 min after addition of TPA. Interestingly, this normalizing TPA effect was not associated with alpha-catenin induction. Classical and confocal immunofluorescence showed only minor TPA-induced changes in E-cadherin staining. In contrast, desmosomal and tight junctional proteins were dramatically rearranged, with a conversion from cytoplasmic clusters to obvious concentration at cell-cell contacts and exposition at the exterior cell surface. Electron microscopical observations revealed the TPA-induced appearance of typical desmosomal plaques. TPA-restored cell-cell adhesion was E-cadherin dependent as demonstrated by a blocking antibody in a cell aggregation assay. Addition of an antibody against the extracellular part of desmoglein-2 blocked the TPA effect, too. Remarkably, the combination of anti-E-cadherin and anti-desmoglein antibodies synergistically inhibited the TPA effect. Our studies show that it is possible to bypass the need for normal alpha-catenin expression to establish tight intercellular adhesion by epithelial cells. Apparently, the underlying mechanism comprises upregulation of desmosomes and tight junctions by activation of the PKC signaling pathway, whereas E-cadherin remains essential for basic cell-cell adhesion, even in the absence of alpha-catenin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.