ArticleThe Biochemical journal1997
NF-kappaB-dependent expression of nitric oxide synthase is required for membrane fusion of chick embryonic myoblasts.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 58 citations in OpenAlex.
- Enhancing health span: muscle stem cells and hormesis.Biogerontology · 2022Review
- nNOS-derived NO modulates force production and iNO-derived NO the excitability in C2C12-derived 3D tissue engineering skeletal muscleFrontiers in physiology · 2022Article
- Role of miR-200c in Myogenic Differentiation Impairment via p66Shc: Implication in Skeletal Muscle Regeneration of DystrophicOxidative medicine and cellular longevity · 2018Article
- Redox Control of Skeletal Muscle Regeneration.Antioxidants & redox signaling · 2017Review
- Structure-guided design of potent diazobenzene inhibitors for the BET bromodomains.Journal of medicinal chemistry · 2013Article
- Reactive oxygen species in skeletal muscle signaling.Journal of signal transduction · 2012Article
- Nitric oxide regulates stretch-induced proliferation in C2C12 myoblasts.Journal of muscle research and cell motility · 2010Article
- NF-kappaB functions in stromal fibroblasts to regulate early postnatal muscle development.The Journal of biological chemistry · 2010Article
- Arginine supplementation induces myoblast fusion via augmentation of nitric oxide production.Journal of muscle research and cell motility · 2006Article
- Role of TNF-{alpha} signaling in regeneration of cardiotoxin-injured muscle.American journal of physiology. Cell physiology · 2005Article
- Postanoxic functional recovery of the developing heart is slightly altered by endogenous or exogenous nitric oxide.Molecular and cellular biochemistry · 2003Article
- Insulin restores differentiation of Ras-transformed C2C12 myoblasts by inducing NF-kappaB through an AKT/P70S6K/p38-MAPK pathway.Oncogene · 2002Article
- Nitric oxide is an essential negative regulator of cell proliferation in Xenopus brain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The activity of nitric oxide synthase (NOS) has recently been shown to increase transiently but dramatically in chick embryonic myoblasts that are competent for fusion and that NO acts as a messenger for the cell fusion. Here we show that the rise in NOS activity is tightly correlated with an increase in NOS protein level, and its synthesis is under transcriptional control. In addition, a partial cDNA sequence of NOS obtained by reverse transcription PCR on total RNA from chick myoblasts was found to be identical with that of the inducible type of NOS (iNOS) from chick macrophage. Thus chick myoblast NOS must belong to the family of iNOS. Consistently, pyrrolidine dithiocarbamate, a potent inhibitor of nuclear factor kappaB (NF-kappaB), prevented the expression of myoblast NOS. Furthermore the antioxidant also strongly inhibited cell fusion, and its inhibitory effect was reversed by treatment with sodium nitroprusside, an NO-generating agent. In addition, nuclear extracts obtained from myoblasts that were competent for fusion, but not those from proliferating cells or from fully differentiated myotubes, were capable of binding to the consensus NF-kappaB site in the promoter region of the gene encoding iNOS. These results suggest that NF-kappaB-dependent expression of NOS is an important step in membrane fusion of chick embryonic myoblasts.
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