ArticleThe Biochemical journal1997
Rat hepatic glutaminase: identification of the full coding sequence and characterization of a functional promoter.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 45 citations in OpenAlex.
- GLS2 Is a Tumor Suppressor and a Regulator of Ferroptosis in Hepatocellular Carcinoma.Cancer research · 2022Article
- Article
- Glutamate and Brain Glutaminases in Drug Addiction.Neurochemical research · 2017Review
- A tale of two glutaminases: homologous enzymes with distinct roles in tumorigenesis.Future medicinal chemistry · 2017Review
- The role of skeletal muscle in liver glutathione metabolism during acetaminophen overdose.Journal of theoretical biology · 2015Article
- Brain slices from glutaminase-deficient mice metabolize less glutamine: a cellular metabolomic study with carbon 13 NMR.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2012Article
- Mammalian glutaminase Gls2 gene encodes two functional alternative transcripts by a surrogate promoter usage mechanism.PloS one · 2012Article
- Novel form of phosphate activated glutaminase in cultured astrocytes and human neuroblastoma cells, PAG in brain pathology and localization in the mitochondria.Neurochemical research · 2008Article
- Segregation of two glutaminase isoforms in islets of Langerhans.The Biochemical journal · 2004Article
- Genomic organization and transcriptional analysis of the human l-glutaminase gene.The Biochemical journal · 2003Article
- Glutaminase isoform expression in cell lines derived from human colorectal adenomas and carcinomas.The Biochemical journal · 2003Article
- Inhibition of glutaminase expression by antisense mRNA decreases growth and tumourigenicity of tumour cells.The Biochemical journal · 2000Article
- Molecular cloning, sequencing and expression studies of the human breast cancer cell glutaminase.The Biochemical journal · 2000Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Glutamine catabolism in mammalian liver is catalysed by a unique isoenzyme of phosphate-activated glutaminase. The full coding and 5' untranslated sequence for rat hepatic glutaminase was isolated by screening lambda ZAP cDNA libraries and a Charon 4a rat genomic library. The sequence produces a mRNA 2225 nt in length, encoding a polypeptide of 535 amino acid residues with a calculated molecular mass of 59.2 kDa. The deduced amino acid sequence of rat liver glutaminase shows 86% similarity to that of rat kidney glutaminase and 65% similarity to a putative glutaminase from Caenorhabditis elegans. A genomic clone to rat liver glutaminase was isolated that contains 3.5 kb of the gene and 7.5 kb of the 5' flanking region. The 1 kb immediately upstream of the hepatic glutaminase gene (from -1022 to +48) showed functional promoter activity in HepG2 hepatoma cells. This promoter region did not respond to treatment with cAMP, but was highly responsive (10-fold stimulation) to the synthetic glucocorticoid dexamethasone. Subsequent 5' deletion analysis indicated that the promoter region between -103 and +48 was sufficient for basal promoter activity. This region does not contain an identifiable TATA element, indicating that transcription of the glutaminase gene is driven by a TATA-less promoter. The region responsive to glucocorticoids was mapped to -252 to -103 relative to the transcription start site.
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