ArticleThe Biochemical journal1997
Identification of membrane dipeptidase as a major glycosyl-phosphatidylinositol-anchored protein of the pancreatic zymogen granule membrane, and evidence for its release by phospholipase A.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Pancreatic glycoprotein 2 is a first line of defense for mucosal protection in intestinal inflammation.Nature communications · 2021Article
- Dipeptidyl Peptidase IV as a Muscle Myokine.Frontiers in physiology · 2020Review
- Regulation of MMP-3 expression and secretion by the chemokine eotaxin-1 in human chondrocytes.Journal of biomedical science · 2011Article
- Is hexamerin receptor a GPI-anchored protein in Achaea janata (Lepidoptera: Noctuidae)?Journal of biosciences · 2011Article
- Dual fluorescent protein reporters for studying cell behaviors in vivo.Genesis (New York, N.Y. : 2000) · 2009Article
- Global topology analysis of pancreatic zymogen granule membrane proteins.Molecular & cellular proteomics : MCP · 2008Article
- Proteomic analysis of pancreatic zymogen granules: identification of new granule proteins.Journal of proteome research · 2007Article
- A novel aminopeptidase in the fat body of the moth Achaea janata as a receptor for Bacillus thuringiensis Cry toxins and its comparison with midgut aminopeptidase.The Biochemical journal · 2007Article
- Endogenous glycosylphosphatidylinositol-specific phospholipase C releases renal dipeptidase from kidney proximal tubules in vitro.The Biochemical journal · 2001Article
- Regulation of brain glycosylphosphatidylinositol-specific phospholipase D by natural amphiphiles.Neurochemical research · 1999Article
- Release of GPI-anchored Zn2+-glycerophosphocholine cholinephosphodiesterase as an amphiphilic form from bovine brain membranes by bee venom phospholipase A2.Neurochemical research · 1999Article
- Efficient binding of regulated secretory protein aggregates to membrane phospholipids at acidic pH.The Biochemical journal · 1999Article
- Sorting and storage during secretory granule biogenesis: looking backward and looking forward.The Biochemical journal · 1998Review
- Insulin stimulates the release of the glycosyl phosphatidylinositol-anchored membrane dipeptidase from 3T3-L1 adipocytes through the action of a phospholipase C.The Biochemical journal · 1997Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membrane dipeptidase (EC 3.4.13.19) enzyme activity that is inhibited by cilastatin has been detected in pancreatic zymogen granule membranes of human, porcine and rat origin. Immunoelectrophoretic blot analysis of human and porcine pancreatic zymogen granule membranes with polyclonal antisera raised against the corresponding kidney membrane dipeptidase revealed that the enzyme is a disulphide-linked homodimer of subunit mass 61 kDa in the human and 45 kDa in the pig. Although membrane dipeptidase was, along with glycoprotein-2, one of the only two major components of carbonate high pH-washed membranes, no enzyme activity or immunoreactivity was detected in the zymogen granule contents. Digestion with bacterial phosphatidylinositol-specific phospholipase C (PI-PLC), and subsequent recognition by antibodies specific for the cross-reacting determinant, revealed that membrane dipeptidase in human and porcine pancreatic zymogen granule membranes is glycosyl-phosphatidylinositol-anchored. Membrane dipeptidase was released from the pancreatic zymogen granule membranes by an endogenous hydrolase, and the released form migrated as a disulphide-linked dimer on SDS/PAGE under non-reducing conditions. Under reducing conditions it migrated with the same apparent molecular mass as the membrane-bound form, and was still a substrate for bacterial PI-PLC. Treatment of kidney microvillar membranes with phospholipase A2 resulted in the release of membrane dipeptidase in a form that demonstrated electrophoretic and cilastatin-Sepharose binding properties identical to those of the endogenously released form of the enzyme from zymogen granule membranes. These results indicate that the glycosyl-phosphatidylinositol anchor on the pancreatic membrane dipeptidase is cleaved by an endogenous hydrolase, probably a phospholipase A, and that this cleavage may promote the release of the protein from the membrane.
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