ArticleJournal of clinical microbiology1997
Determination of genotypes of Toxoplasma gondii strains isolated from patients with toxoplasmosis.
Article in Journal of clinical microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 141 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
141 citing papers in PubMed, 2 syntheses or guidelines pooled it, 513 citations in OpenAlex.
- A systematic review on efficiency of microneme proteins to induce protective immunity against Toxoplasma gondii.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Pooled it
- Is Toxoplasma gondii type related to clinical outcome in human congenital infection? Systematic and critical review.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Pooled it
- Endogenous cathelicidin protects againstInfection and immunity · 2026Article
- Seroprevalence and Genotype Distribution ofHealth science reports · 2026Article
- Elevated Liver Enzymes and Genotype I of T. gondii Among Liver Cancer Patients: Evidence of a Potential Link.Asian Pacific journal of cancer prevention : APJCP · 2026Article
- Genotype distribution and risk factors of Toxoplasma gondii infection in animals of Trishal, Bangladesh.PloS one · 2026Article
- Evolution ofJournal of parasitology research · 2026Review
- Direct genotype analysis of Toxoplasma gondii in ocular samples from patients with active rtinochoroiditis.BMC infectious diseases · 2025Article
- Detection ofInternational journal for parasitology. Parasites and wildlife · 2025Article
- PCR Detection, Genotyping, and Differentiation ofPathogens (Basel, Switzerland) · 2025Article
- Causes of mortality and morbidity in the endangered southern brown bandicoot (Isoodon obesulus obesulus).Australian veterinary journal · 2025Article
- Sero-molecular prevalence of toxoplasmosis in hemodialysis and peritoneal dialysis patients in Markazi Province, Iran.Tropical medicine and health · 2025Article
- Toxoplasma gondii infection supports the infiltration of T cells into brain tumors.Journal of neuroimmunology · 2024Article
- Effective factors in the pathogenesis ofHeliyon · 2024Review
- Presence of IgG antibodies is not a reliable marker ofInternational journal for parasitology. Parasites and wildlife · 2023Article
- Serotyping, a challenging approach forFrontiers in medicine · 2023Review
- Establishment of a murine model of congenital toxoplasmosis and validation of a qPCR assay to assess the parasite load in maternal and fetal tissues.Frontiers in microbiology · 2023Article
- Parallel detection of multiple zoonotic parasites using a real-time fluorogenic loop-mediated isothermal amplification-based quadruple-sample microfluidic chip.Frontiers in microbiology · 2023Article
- Genotyping of Toxoplasma gondii Strains from Goats in Jahrom District, Southern Iran.Acta parasitologica · 2022Article
- Human MHC class I molecule, HLA-A2.1, mediates activation of CD8Frontiers in immunology · 2022Article
81 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
To determine the genotypes of Toxoplasma gondii strains associated with human toxoplasmosis, we developed a sensitive approach for typing parasites grown from clinical samples by short-term in vitro culture. A newly described nested PCR assay was capable of amplifying genomic DNA from as few as five parasites in the presence of host tissues. Typing was based on DNA polymorphisms at the SAG2 locus, encoding tachyzoite surface antigen p22. Restriction fragment length polymorphisms in PCR-amplified SAG2 products were used to classify strains into one of the three major lineages of T. gondii. This approach was successfully used to determine the genotypes of 68 of 72 samples that had been previously isolated from patients with congenital, cerebral, and disseminated toxoplasmosis. Type II strains of T. gondii were found in a majority of the samples, accounting for 55 (81%) of the 68 toxoplasmosis cases. In contrast, type I and III strains were found in only 7 (10%) and 6 (9%) of the 68 cases, respectively. The results of this study support the previous finding that type II strains are most often associated with human toxoplasmosis. Nested PCR analysis at the SAG2 locus provides rapid assignment of T. gondii to a specific genotype that should be useful in analyzing a variety of clinical samples.
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Registered trials
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