ArticleJournal of clinical microbiology1997
Rapid identification and fingerprinting of Candida krusei by PCR-based amplification of the species-specific repetitive polymorphic sequence CKRS-1.
Article in Journal of clinical microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Random amplification of polymorphic DNA and microsatellite genotyping of pre- and posttreatment isolates of Candida spp. from human immunodeficiency virus-infected patients on different fluconazole regimens.Journal of clinical microbiology · 1998Trial
- Development of a Multiplex PCR Short Tandem Repeat Typing Scheme for Candida krusei.Journal of clinical microbiology · 2022Article
- Mechanisms of AcquiredAntimicrobial agents and chemotherapy · 2020Article
- Current Aspects in the Biology, Pathogeny, and Treatment ofInfection and drug resistance · 2020Review
- Molecular Strain Typing of Clinical Isolates,Journal of clinical and diagnostic research : JCDR · 2017Article
- Rapid development of Candida krusei echinocandin resistance during caspofungin therapy.Antimicrobial agents and chemotherapy · 2015Article
- Variation in genotype and higher virulence of a strain of Sporothrix schenckii causing disseminated cutaneous sporotrichosis.Mycopathologia · 2011Article
- Genotyping of Trichophyton rubrum by analysis of ribosomal-DNA intergenic spacer regions.Mycopathologia · 2007Article
- Candida krusei transmission among hematology patients resolved by adapted antifungal prophylaxis and infection control measures.Journal of clinical microbiology · 2006Article
- Comparison of PCR- and HinfI restriction endonuclease-based methods for typing of Candida krusei isolates.Journal of clinical microbiology · 2004Article
- Restriction fragment length polymorphism analysis of ribosomal DNA intergenic regions is useful for differentiating strains of Trichophyton mentagrophytes.Journal of clinical microbiology · 2003Article
- Strain identification of Trichophyton rubrum by specific amplification of subrepeat elements in the ribosomal DNA nontranscribed spacer.Journal of clinical microbiology · 2000Article
- Molecular differentiation of seven Malassezia species.Journal of clinical microbiology · 2000Article
- Clonal and spontaneous origins of fluconazole resistance in Candida albicans.Journal of clinical microbiology · 2000Article
- Species and genotypic diversities and similarities of pathogenic yeasts colonizing women.Journal of clinical microbiology · 1999Article
- Molecular markers reveal exclusively clonal reproduction in Trichophyton rubrum.Journal of clinical microbiology · 1999Article
- Specific detection of fusarium species in blood and tissues by a PCR technique.Journal of clinical microbiology · 1999Article
- Species identification and strain differentiation of dermatophyte fungi by analysis of ribosomal-DNA intergenic spacer regions.Journal of clinical microbiology · 1999Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A PCR method was developed to identify and fingerprint Candida krusei isolates simply and rapidly. The primer pair Arno1 and Arno2 was designed to amplify the polymorphic species-specific repetitive sequence CKRS-1 (C. krusei repeated sequence 1) that we identified in the nontranscribed intergenic regions (IGRs) of rRNA genes in C. krusei LMCK31. The specificity, sensitivity, reproducibility, and fingerprinting ability of the PCR assay were evaluated. Amplification products were obtained from all 131 C. krusei isolates studied. No other yeast species of medical importance (n = 26), including species similar to C. krusei, species of pathogenic filamentous fungi, or a variety of pathogenic bacteria, yielded a PCR product with these primers. This PCR assay allowed for the identification of C. krusei in less than 6 h. The PCR assay was sensitive enough to detect as little as 10 to 100 fg of C. krusei-purified DNA and proved to be reproducible. Since amplification products varied both in number and in molecular weight according to the strains, PCR patterns allowed strains to be distinguished. To ascertain the epidemiological usefulness of this PCR fingerprinting, the patterns of the 131 isolates were compared. A total of 95 types which corresponded to 95 independent strains were delineated (discriminatory power = 1 with n = 95). Comparison of the results of PCR fingerprinting and those of fingerprinting with the CkF1,2 probe showed that they concurred. In addition, this work yields insights into the mechanisms involved in generating polymorphisms in the IGRs of C. krusei. Since this method is simpler and faster than established identification and genotyping methods of this important pathogenic species, it is a critical improvement for clinical microbiology laboratories relevant not only to diagnosis but also to epidemiology.
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