ArticlePlant physiology1997
Characterization of AtSEC12 and AtSAR1. Proteins likely involved in endoplasmic reticulum and Golgi transport.
Article in Plant physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 127 citations in OpenAlex.
- A Nitrogen-specific Interactome Analysis Sheds Light on the Role of the SnRK1 and TOR Kinases in Plant Nitrogen Signaling.Molecular & cellular proteomics : MCP · 2024Article
- A cargo sorting receptor mediates chloroplast protein trafficking through the secretory pathway.The Plant cell · 2024Article
- Point mutations in the catalytic domain disrupt cellulose synthase (CESA6) vesicle trafficking and protein dynamics.The Plant cell · 2023Article
- RPG interacts with E3-ligase CERBERUS to mediate rhizobial infection in Lotus japonicus.PLoS genetics · 2023Article
- A deleterious Sar1c variant in rice inhibits export of seed storage proteins from the endoplasmic reticulum.Plant molecular biology · 2023Article
- The EXO70 inhibitor Endosidin2 alters plasma membrane protein composition in Arabidopsis roots.Frontiers in plant science · 2023Article
- Identification of a defense response gene involved in signaling pathways against PVA and PVY in potato.GM crops & food · 2021Article
- The plant endoplasmic reticulum: an organized chaos of tubules and sheets with multiple functions.Journal of microscopy · 2020Review
- Endosidin20 Targets the Cellulose Synthase Catalytic Domain to Inhibit Cellulose Biosynthesis.The Plant cell · 2020Article
- Transport from the endoplasmic reticulum to the Golgi in plants: Where are we now?Seminars in cell & developmental biology · 2018Review
- Expression of the Intracellular COPT3-Mediated Cu Transport Is Temporally Regulated by the TCP16 Transcription Factor.Frontiers in plant science · 2018Article
- Differential Proteomic Analysis Reveals the Effect of Calcium on Malus baccata Borkh. Leaves under Temperature Stress.International journal of molecular sciences · 2017Article
- GTPase ROP2 binds and promotes activation of target of rapamycin, TOR, in response to auxin.The EMBO journal · 2017Article
- Composition, Assembly, and Trafficking of a Wheat Xylan Synthase Complex.Plant physiology · 2016Article
- Unique COPII component AtSar1a/AtSec23a pair is required for the distinct function of protein ER export in Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Vesicles versus Tubes: Is Endoplasmic Reticulum-Golgi Transport in Plants Fundamentally Different from Other Eukaryotes?Plant physiology · 2015Review
- Heterodimeric capping protein from Arabidopsis is a membrane-associated, actin-binding protein.Plant physiology · 2014Article
- A proteomic strategy for global analysis of plant protein complexes.The Plant cell · 2014Article
- Article
- Study of the plant COPII vesicle coat subunits by functional complementation of yeast Saccharomyces cerevisiae mutants.PloS one · 2014Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transport of cargo proteins from the endoplasmic reticulum (ER) to the cis-Golgi network is mediated by protein-coated vesicles. The coat, called COPII coat, consists of proteins that are recruited from the cytosol and interact with integral membrane proteins of the ER. In yeast, both cytosolic proteins (Sec13/31, Sec23/24, and Sar1) and ER-associated proteins (Sec12 and others) have been purified and characterized and it has been possible to demonstrate transport vesicle formation in vitro. Arabidopsis thaliana homologs of Sar1 and Sec12 have recently been identified, but little is known about the properties of the proteins or their subcellular distribution. Here we demonstrate that AtSAR1, a 22-kD protein that binds GTP, and AtSEC12, a 43-kD GTP-exchange protein, are both associated with the ER. However, about one-half of the cellular AtSAR1 is present in the cytosol. When AtSAR1 is overexpressed in transgenic plants, the additional protein is also cytosolic. When tissue-culture cells are cold-shocked (12 h at 8 degrees C), AtSAR1 levels appeared to decline and a larger proportion of the total protein was found in the cytosol. Given the known function of AtSAR1 in yeast, we propose that the amount of ER-associated AtSAR1 is an indication of the intensity of the secretory process. Thus, we expect that such a cold shock will adversely affect ER-to-Golgi transport of proteins.
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