ArticleProceedings of the National Academy of Sciences of the United States of America1997
Human BRCA1 inhibits growth in yeast: potential use in diagnostic testing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 105 citations in OpenAlex.
- Candidiasis in breast cancer: Tumor progression or not?Iranian journal of basic medical sciences · 2024Review
- ExploringMicrobial cell (Graz, Austria) · 2021Review
- Article
- Yeast-based assays for the functional characterization of cancer-associated variants of human DNA repair genes.Microbial cell (Graz, Austria) · 2020Review
- Functional investigation of theMolecular biology research communications · 2019Article
- Review
- Expression of cancer related BRCA1 missense variants decreases MMS-induced recombination in Saccharomyces cerevisiae without altering its nuclear localization.Cell cycle (Georgetown, Tex.) · 2016Article
- Functional Assessment of Genetic Variants with Outcomes Adapted to Clinical Decision-Making.PLoS genetics · 2016Article
- BRCA1 Circos: a visualisation resource for functional analysis of missense variants.Journal of medical genetics · 2015Article
- Article
- Missense variants of uncertain significance (VUS) altering the phosphorylation patterns of BRCA1 and BRCA2.PloS one · 2013Article
- A guide for functional analysis of BRCA1 variants of uncertain significance.Human mutation · 2012Review
- Functional analyses of human DNA repair proteins important for aging and genomic stability using yeast genetics.DNA repair · 2012Review
- Profiling of the BRCA1 transcriptome through microarray and ChIP-chip analysis.Nucleic acids research · 2011Article
- Yeast two-hybrid and itc studies of alpha and beta spectrin interaction at the tetramerization site.Cellular & molecular biology letters · 2011Article
- Multifunctional transcription factor TFII-I is an activator of BRCA1 function.British journal of cancer · 2011Article
- Identification of DBC1 as a transcriptional repressor for BRCA1.British journal of cancer · 2010Article
- Analysis of a set of missense, frameshift, and in-frame deletion variants of BRCA1.Mutation research · 2009Article
- Positional analyses of BRCA1-dependent expression in Saccharomyces cerevisiae.Cell cycle (Georgetown, Tex.) · 2008Article
- Assessment of functional effects of unclassified genetic variants.Human mutation · 2008Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Germline-inactivating mutations of BRCA1 result in a hereditary predisposition to breast and ovarian cancer. Truncating mutations of BRCA1 predispose to cancer and can be ascertained by protein truncation testing or sequencing. However, cancer-predisposing missense mutations of BRCA1 are difficult to distinguish from polymorphisms by genetic testing methods currently used. Here we show that expression of BRCA1 or BRCA1 fused to a GAL4 activation domain in Saccharomyces cerevesiae inhibits growth, resulting in small colonies easily distinguishable from vector-transformed controls. The growth inhibitory effect can be localized to sequences encoding the recently described BRCA1 C-terminal domains. Growth suppression by a BRCA1 fusion protein is not influenced by introduction of neutral polymorphisms but is diminished or abolished by frameshift, nonsense, or disease-associated missense mutations located in the C-terminal 305 amino acids of BRCA1. These observations may permit the functional significance of many BRCA1 sequence changes to be assessed in yeast. Additionally, the correlation of growth suppression with wild-type forms of BRCA1 suggests that the assay may be capable of detecting functionally conserved interactions between the evolutionarily conserved BRCA1 C-terminal domains and cellular elements found in both human and yeast cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.