ArticleProceedings of the National Academy of Sciences of the United States of America1997
In situ molecular association of dystrophin with actin revealed by sensitized emission immuno-resonance energy transfer.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 39 citations in OpenAlex.
- Cy3-ATP labeling of unfixed, permeabilized mouse hair cells.Scientific reports · 2021Article
- Close proximity of myosin loop 3 to troponin determined by triangulation of resonance energy transfer distance measurements.Biochemistry · 2009Article
- Parkin localizes to the Lewy bodies of Parkinson disease and dementia with Lewy bodies.The American journal of pathology · 2002Article
- Engineering a terbium-binding site into an integral membrane protein for luminescence energy transfer.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Long-wavelength long-lifetime luminophores.Analytical chemistry · 2001Article
- A close association of torsinA and alpha-synuclein in Lewy bodies: a fluorescence resonance energy transfer study.The American journal of pathology · 2001Article
- Flow (shear stress)-induced endothelium-dependent dilation is altered in mice lacking the gene encoding for dystrophin.Circulation · 2001Article
- On the possibility of long-wavelength long-lifetime high-quantum-yield luminophores.Analytical biochemistry · 2001Article
- Conformational selection during weak binding at the actin and myosin interface.Biophysical journal · 2000Article
- Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein.Journal of virology · 1998Article
- Temporally and spectrally resolved imaging microscopy of lanthanide chelates.Biophysical journal · 1998Article
- Luminescence resonance energy transfer measurements in myosin.Biophysical journal · 1998Article
- Synthesis of fluorescent analogs of alpha-conotoxin MII.Bioconjugate chemistryArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel method was developed to detect molecular associations of dystrophin with actin in cryostat muscle tissue sections by combining resonance energy transfer technology with immunohistochemical techniques. This method takes advantage of the long phosphorescent lifetime of terbium chelates, a property that enables the accurate determination of energy transfer in biological tissues by lifetime measurements of sensitized emission. After a brief excitation pulse, terbium chelates emit for milliseconds after the intrinsically high autofluorescence of biological specimens has decayed to negligible levels. Rat skeletal muscle tissue sections were labeled with both anti-dystrophin monoclonal antibody conjugated to a terbium-based resonance energy transfer donor and anti-actin tetramethylrhodamine phalloidin as an acceptor. Resonance energy transfer between the two probes indicated that the distance separating the probes is within 10 nm (about the size of an IgG2b antibody molecule). The fraction of antibodies that participated in resonance energy transfer was estimated to be 80-90% because of the close agreement between the quenching of donor phosphorescence and the efficiency of resonance energy transfer revealed by lifetime measurements of sensitized emission by tetramethyl-rhodamine phalloidin. Sensitized emission was detectable only when both anti-dystrophin antibody and tetramethyl-rhodamine phalloidin were present. These results indicate that actin and dystrophin are closely associated within the cell. This method is potentially applicable to the investigation of many types of intracellular associations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.