ArticleProceedings of the National Academy of Sciences of the United States of America1997
Messenger RNA deadenylylation precedes decapping in mammalian cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
105 citing papers in PubMed, 206 citations in OpenAlex.
- The Incoming Influenza Genome Assembles a Host RBP Network that Orchestrates Viral RNA Synthesis.bioRxiv : the preprint server for biology · 2025Article
- The Mysterious World of Non-Canonical Caps - What We Know and Why We Need New Sequencing Techniques.Chembiochem : a European journal of chemical biology · 2025Review
- An extended wave of global mRNA deadenylation sets up a switch in translation regulation across the mammalian oocyte-to-embryo transition.Cell reports · 2024Article
- Future Perspectives for the Identification and Sequencing of Nicotinamide Adenine Dinucleotide-Capped RNAs.Accounts of chemical research · 2023Article
- NADcapPro and circNC: methods for accurate profiling of NAD and non-canonical RNA caps in eukaryotes.Communications biology · 2023Article
- Measuring the tail: Methods for poly(A) tail profiling.Wiley interdisciplinary reviews. RNA · 2023Review
- A NYN domain protein directly interacts with DECAPPING1 and is required for phyllotactic pattern.Plant physiology · 2022Article
- Exploring Translational Control of Maternal mRNAs in Zebrafish.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Establishment of 5'-3' interactions in mRNA independent of a continuous ribose-phosphate backbone.RNA (New York, N.Y.) · 2020Article
- Functional Characterization of a Dual Enhancer/Promoter Regulatory Element Leading HumanFrontiers in genetics · 2020Article
- RNA degradomes reveal substrates and importance for dark and nitrogen stress responses of Arabidopsis XRN4.Nucleic acids research · 2019Article
- Inhibition of Poly(A)-binding protein with a synthetic RNA mimic reduces pain sensitization in mice.Nature communications · 2018Article
- RRP42, a Subunit of Exosome, Plays an Important Role in Female Gametophytes Development and Mesophyll Cell Morphogenesis inFrontiers in plant science · 2017Article
- Oligoadenylation of 3' decay intermediates promotes cytoplasmic mRNA degradation in Drosophila cells.RNA (New York, N.Y.) · 2016Article
- Mobilization of Dormant Cnot7 mRNA Promotes Deadenylation of Maternal Transcripts During Mouse Oocyte Maturation.Biology of reproduction · 2015Article
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- Biogenesis, turnover, and mode of action of plant microRNAs.The Plant cell · 2013Review
- RRP41L, a putative core subunit of the exosome, plays an important role in seed germination and early seedling growth in Arabidopsis.Plant physiology · 2013Article
- Translational repression precedes and is required for ZAP-mediated mRNA decay.The EMBO journal · 2012Article
45 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
In yeast, the major mRNA degradation pathway is initiated by poly(A) tail shortening that triggers mRNA decapping. The mRNA is then degraded by 5'-to-3' exonucleolysis. In mammalian cells, even though poly(A) tail shortening also precedes mRNA degradation, the degradation pathway has not been elucidated. We have used a reverse transcription-PCR approach that relies on mRNA circularization to measure the poly(A) tail length of four mammalian mRNAs. This approach allows for the simultaneous analysis of the 5' and 3' ends of the same mRNA molecule. For all four mRNAs analyzed, this strategy permitted us to demonstrate the existence of small amounts of decapped mRNA species which have a shorter poly(A) tail than their capped counterparts. Kinetic analysis of one of these mRNAs indicates that the decapped species with a short poly(A) tail are mRNA degradation products. Therefore, our results indicate that decapping is preceded by a shortening of the poly(A) tail in mammalian cells, as it is in yeast, suggesting that this mRNA degradation pathway is conserved throughout eukaryotic evolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.