ArticleGut1997
The trophic action of growth hormone, insulin-like growth factor-I, and insulin on human duodenal mucosa cultured in vitro.
Article in Gut, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Pediatric enteral nutrition therapy for burn victims: when should it be initiated?Revista Brasileira de terapia intensiva · 2019Pooled it
- Effects of recombinant human growth hormone on enterocutaneous fistula patients.World journal of gastroenterology · 2008Trial
- Effects of Cholinergic and Opioid Antagonists on In Vitro Release of Met-Enkephalin, Somatostatin and Insulin-like Growth Factor-1 by and PENK Expression in Crop, Proventriculus and Duodenum of Newly Hatched Chickens.Animals : an open access journal from MDPI · 2025Article
- Intestinal Microbiota Dysbiosis Role and Bacterial Translocation as a Factor for Septic Risk.International journal of molecular sciences · 2025Review
- A nanoemulsion/micelles mixed nanosystem for the oral administration of hydrophobically modified insulin.Drug delivery and translational research · 2021Article
- Influence of Growth Hormone and Glutamine on Intestinal Stem Cells: A Narrative Review.Nutrients · 2019Review
- Adult zebrafish intestine resection: a novel model of short bowel syndrome, adaptation, and intestinal stem cell regeneration.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- The role of growth factors in intestinal regeneration and repair in necrotizing enterocolitis.Seminars in pediatric surgery · 2013Review
- The role of hyperglycemia in burned patients: evidence-based studies.Shock (Augusta, Ga.) · 2010Review
- Effect of growth hormone on small intestinal homeostasis relation to cellular mediators IGF-I and IGFBP-3.World journal of gastroenterology · 2009Article
- Intestinal adaptation after massive intestinal resection.Postgraduate medical journal · 2005Review
- Alterations of growth hormone, cortisol, luteinizing hormone, and insulin concentrations in early-postnatal calves affected with diarrhea.Domestic animal endocrinology · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExperimental evidence suggests that hormones may regulate small intestinal adaptation after surgical resection.
aimsTo characterise the effect of recombinant human growth hormone (GH), insulin-like growth factor-I (IGF-I) and insulin on crypt epithelial cell proliferation in the human duodenal mucosa cultured in vitro. PATIENTS: Thirty nine adults had endoscopic duodenal biopsy specimens taken, which were histologically normal and pair matched specimens from each patient acted as their own control.
methodsPaired biopsy specimens from patients were cultured in vitro, with or without the addition of GH (0.004 IU/ml), IGF-I (400 ng/ml) or insulin (50 micrograms/ml), alone or in combination. After 22 hours, vincristine sulphate was added to the cultures and three hours later specimens were removed and fixed, and DNA stained by the Feulgen method. Intestinal crypts were microdissected and crypt epithelial cell proliferation determined by estimating mean numbers of accumulated metaphase arrests/crypt present in tests and controls between 22-25 hours of culture.
resultsThe addition of GH, IGF-I, and insulin, alone or in combination, significantly increased crypt epithelial cell proliferation in test explants compared with controls. IGF-I was most potent and its trophic effect was modified by insulin.
conclusionsGH, IGF-I, and insulin are involved in the regulation of crypt cell proliferation in the human small intestine in vitro and possibly in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.