ArticleThe EMBO journal1997
D-myo-inositol 1,4,5-trisphosphate 3-kinase A is activated by receptor activation through a calcium:calmodulin-dependent protein kinase II phosphorylation mechanism.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 55 citations in OpenAlex.
- A Class I HDAC Inhibitor BG45 Alleviates Cognitive Impairment through the CaMKII/ITPKA/CaPharmaceuticals (Basel, Switzerland) · 2022Article
- Genome-wide DNA methylation changes associated with olfactory learning and memory in Apis mellifera.Scientific reports · 2017Article
- Inositol 1,4,5-trisphosphate 3-kinase A is a novel microtubule-associated protein: PKA-dependent phosphoregulation of microtubule binding affinity.The Journal of biological chemistry · 2012Article
- Computational quest for understanding the role of astrocyte signaling in synaptic transmission and plasticity.Frontiers in computational neuroscience · 2012Article
- Inositol trisphosphate 3-kinases: focus on immune and neuronal signaling.Cellular and molecular life sciences : CMLS · 2010Review
- Glutamate regulation of calcium and IP3 oscillating and pulsating dynamics in astrocytes.Journal of biological physics · 2009Article
- Acetylcholine-induced Ca2+ oscillations are modulated by a Ca2+ regulation of InsP3R2 in rat portal vein myocytes.Pflugers Archiv : European journal of physiology · 2008Article
- Models of IP3 and Ca2+ oscillations: frequency encoding and identification of underlying feedbacks.Biophysical journal · 2006Article
- The regulation and function of inositol 1,4,5-trisphosphate 3-kinases.Advances in enzyme regulation · 2006Article
- Inositol (1,4,5) trisphosphate 3 kinase B controls positive selection of T cells and modulates Erk activity.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Simulations of inositol phosphate metabolism and its interaction with InsP(3)-mediated calcium release.Biophysical journal · 2002Article
- Article
- Effects of elevated expression of inositol 1,4,5-trisphosphate 3-kinase B on Ca2+ homoeostasis in HeLa cells.The Biochemical journal · 2000Article
- Cloning and expression of a cDNA encoding human inositol 1,4,5-trisphosphate 3-kinase C.The Biochemical journal · 2000Article
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3 authors.
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Abstract
D-myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] 3-kinase, the enzyme responsible for production of D-myo-inositol 1,3,4,5-tetrakisphosphate, was activated 3- to 5-fold in homogenates of rat brain cortical slices after incubation with carbachol. The effect was reproduced in response to UTP in Chinese hamster ovary (CHO) cells overexpressing Ins(1,4,5)P3 3-kinase A, the major isoform present in rat and human neuronal cells. In ortho-32P-labelled cells, the phosphorylated 53 kDa enzyme could be identified after receptor activation by immunoprecipitation. The time course of phosphorylation was very similar to that observed for carbachol (or UTP)-induced enzyme activation. Enzyme phosphorylation was prevented in the presence of okadaic acid. Calmodulin (CaM) kinase II inhibitors (i.e. KN-93 and KN-62) prevented phosphorylation of Ins(1,4,5)P3 3-kinase. Identification of the phosphorylation site in transfected CHO cells indicated that the phosphorylated residue was Thr311. This residue of the human brain sequence lies in an active site peptide segment corresponding to a CaM kinase II-mediated phosphorylation consensus site, i.e. Arg-Ala-Val-Thr. The same residue in Ins(1,4,5)P3 3-kinase A was also phosphorylated in vitro by CaM kinase II. Phosphorylation resulted in 8- to 10-fold enzyme activation and a 25-fold increase in sensitivity to the Ca2+:CaM complex. In this study, direct evidence is provided for a novel regulation mechanism for Ins(1,4,5)P3 3-kinase (isoform A) in vitro and in intact cells.
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