ArticlePlant molecular biology1997
High content, size and distribution of single-stranded DNA in the mitochondria of Chenopodium album (L.).
Article in Plant molecular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 39 citations in OpenAlex.
- Professor Thomas Börner: the pioneer of molecular plant genetics in East Germany turns 80!Molecular genetics and genomics : MGG · 2026Article
- Three Parts of the Plant Genome: On the Way to Success in the Production of Recombinant Proteins.Plants (Basel, Switzerland) · 2022Review
- DNA Repair and the Stability of the Plant Mitochondrial Genome.International journal of molecular sciences · 2020Review
- Tailor-Made Detection of Individual Phosphorylated and Non-Phosphorylated EPIYA-Motifs ofCancers · 2019Article
- Changes in DNA damage, molecular integrity, and copy number for plastid DNA and mitochondrial DNA during maize development.Journal of experimental botany · 2014Article
- A conserved lysine residue of plant Whirly proteins is necessary for higher order protein assembly and protection against DNA damage.Nucleic acids research · 2012Article
- Characterization of the structure and DNA complexity of mung bean mitochondrial nucleoids.Molecules and cells · 2011Article
- Overexpression of mtDNA-associated AtWhy2 compromises mitochondrial function.BMC plant biology · 2008Article
- Arabidopsis phage-type RNA polymerases: accurate in vitro transcription of organellar genes.The Plant cell · 2007Article
- Characterization of a mitochondrially targeted single-stranded DNA-binding protein in Arabidopsis thaliana.Molecular genetics and genomics : MGG · 2005Article
- Mitochondrial DNA heteroplasmy in wheat, Aegilops and their nucleus-cytoplasm hybrids.Genetics · 2002Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondrial (mt) DNA of higher plants is unique in its large size and complexity. We report here a hitherto unknown feature, the presence of large quantities of single-stranded (ss) DNA. About 2.0-8.5% of the chromosomal mtDNA from a suspension culture (depending on the growth stage) and 6.5% of the chromosomal mtDNA from whole plants of Chenopodium album were found to be in ss form by dot-blot hybridization after neutral transfer. Similar amounts of ss mtDNA were observed by binding of the single-strand binding (SSB) protein of Escherichia coli under the electron microscope. Significantly less ssDNA was found in plastids of C. album and in E. coli cells. We observed ss regions between 100 and 22,800 bases distributed in the mt genome spaced from 0.5-100 kb apart. After pulsed-field gel electrophoresis (PFGE), the well-bound fraction of mtDNA (found to consist of circular, sigma-shaped and rosette-like molecules), contained the major part of ssDNA as opposed to the migrating linear molecules. Digestion of mtDNA by ss-specific nucleases followed by PFGE mobilized all well-bound DNA and correspondingly increased the quantity of migrating linear DNA molecules. The implications of ssDNA for the structural organization on plant mt genomes are discussed.
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