ArticlePlant molecular biology1997
Enhanced expression in tobacco of the gene encoding green fluorescent protein by modification of its codon usage.
Article in Plant molecular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 131 citations in OpenAlex.
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- Complete chloroplast genome of Lilium ledebourii (Baker) Boiss and its comparative analysis: lights into selective pressure and adaptive evolution.Scientific reports · 2022Article
- Analysis of Codon Usage Patterns inGenes · 2021Article
- Uncovering dynamic evolution in the plastid genome of seven Ligusticum species provides insights into species discrimination and phylogenetic implications.Scientific reports · 2021Article
- Chlorinated Auxins-How DoesInternational journal of molecular sciences · 2020Article
- Protein trap: a new Swiss army knife for geneticists?Molecular biology reports · 2020Review
- Comparative chloroplast genomes of Paris Sect. Marmorata: insights into repeat regions and evolutionary implications.BMC genomics · 2018Article
- Green Fluorescent Protein Expression in Pseudogymnoascus destructans to Study Its Abiotic and Biotic Lifestyles.Mycopathologia · 2018Article
- A simple and efficient CRISPR/Cas9 platform for induction of single and multiple, heritable mutations in barley (Plant methods · 2018Article
- A hyper-thermostable α-amylase from Pyrococcus furiosus accumulates in Nicotiana tabacum as functional aggregates.BMC biotechnology · 2017Article
- Analysis of transcriptome data reveals multifactor constraint on codon usage in Taenia multiceps.BMC genomics · 2017Article
- Chloroplast Genome Sequence of Pigeonpea (Frontiers in plant science · 2016Article
- Anti-infective immunoadhesins from plants.Plant biotechnology journal · 2015Review
- Complete Chloroplast Genome of the Wollemi Pine (Wollemia nobilis): Structure and Evolution.PloS one · 2015Article
- Proof by synthesis of Tobacco mosaic virus.Genome biology · 2014Article
- Synthetic versions of firefly luciferase and Renilla luciferase reporter genes that resist transgene silencing in sugarcane.BMC plant biology · 2014Article
- Characterization of VRC01, a potent and broadly neutralizing anti-HIV mAb, produced in transiently and stably transformed tobacco.Plant biotechnology journal · 2014Article
- Single-molecule detection and tracking in plants.Protoplasma · 2014Review
- Article
- Fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants.Frontiers in plant science · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene encoding green fluorescent protein (GFP) from Aequorea victoria was resynthesized to adapt its codon usage for expression in plants by increasing the frequency of codons with a C or a G in the third position from 32 to 60%. The strategy for constructing the synthetic gfp gene was based on the overlap extension PCR method using 12 long oligonucleotides as the starting material and as primers. The new gene contains 101 silent nucleotide changes compared to its wild-type counterpart used in this study. Several transgenic tobacco lines containing the wild-type gfp gene contained minute amounts of a smaller protein cross-reacting with GFP antiserum, whereas only one protein of the expected size was found in transgenics with the synthetic gfp gene. The smaller protein was probably encoded by a truncated gfp mRNA created by splicing of a 84 bp cryptic intron as detected by a reverse transcription-PCR technique. A comparison of GFP production in transgenics with the wild-type and the synthetic gfp gene under the control of the enhanced CaMV 35S promoter showed that the large-scale alterations in the gfp gene increased the frequency of high expressors in the transgenic population but hardly changed the maximum GFP concentrations. The latter phenomenon may be attributed to a reduced regeneration capacity of transformed cells with higher GFP concentrations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.