ArticleMolecular and cellular biology1997
A ubiquitin-conjugating enzyme in fission yeast that is essential for the onset of anaphase in mitosis.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
- UBE2C overexpression in melanoma and its essential role in G2/M transition.Journal of Cancer · 2019Article
- The zinc-binding region (ZBR) fragment of Emi2 can inhibit APC/C by targeting its association with the coactivator Cdc20 and UBE2C-mediated ubiquitylation.FEBS open bio · 2014Article
- An E2 enzyme Ubc11 is required for ubiquitination of Slp1/Cdc20 and spindle checkpoint silencing in fission yeast.Cell cycle (Georgetown, Tex.) · 2013Article
- lemmingA encodes the Apc11 subunit of the APC/C in Drosophila melanogaster that forms a ternary complex with the E2-C type ubiquitin conjugating enzyme, Vihar and Morula/Apc2.Cell division · 2012Article
- Processive ubiquitin chain formation by the anaphase-promoting complex.Seminars in cell & developmental biology · 2011Review
- Nuclear protein quality is regulated by the ubiquitin-proteasome system through the activity of Ubc4 and San1 in fission yeast.The Journal of biological chemistry · 2011Article
- State of the APC/C: organization, function, and structure.Critical reviews in biochemistry and molecular biology · 2011Review
- APC16 is a conserved subunit of the anaphase-promoting complex/cyclosome.Journal of cell science · 2010Article
- UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit.Nature cell biology · 2009Article
- Requirements for protein phosphorylation and the kinase activity of polo-like kinase 1 (Plk1) for the kinetochore function of mitotic arrest deficiency protein 1 (Mad1).The Journal of biological chemistry · 2008Article
- The unique N terminus of the UbcH10 E2 enzyme controls the threshold for APC activation and enhances checkpoint regulation of the APC.Molecular cell · 2008Article
- Rapid regulation of protein activity in fission yeast.BMC cell biology · 2008Article
- Two ubiquitin-conjugating enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclin.Molecular and cellular biology · 2003Article
- Molecular characterization of plant ubiquitin-conjugating enzymes belonging to the UbcP4/E2-C/UBCx/UbcH10 gene family.Plant physiology · 2002Article
- The fission yeast ubiquitin-conjugating enzymes UbcP3, Ubc15, and Rhp6 affect transcriptional silencing of the mating-type region.Eukaryotic cell · 2002Article
- APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex.Molecular biology of the cell · 2001Article
- Cell cycle-dependent expression of mammalian E2-C regulated by the anaphase-promoting complex/cyclosome.Molecular biology of the cell · 2000Article
- The APC11 RING-H2 finger mediates E2-dependent ubiquitination.Molecular biology of the cell · 2000Article
- Cell cycle-regulated proteolysis of mitotic target proteins.Molecular biology of the cell · 1999Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA encoding a ubiquitin-conjugating enzyme designated UbcP4 in fission yeast was isolated. Disruption of its genomic gene revealed that it was essential for cell viability. In vivo depletion of the UbcP4 protein demonstrated that it was necessary for cell cycle progression at two phases, G2/M and metaphase/anaphase transitions. The G2 arrest of UbcP4-depleted cells was dependent upon chk1, which mediates checkpoint pathway. UbcP4-depleted cells arrested at metaphase had condensed chromosomes but were defective in separation. However, septum formation and cytokinesis were not restrained during the metaphase arrest. Overexpression of UbcP4 specifically rescued the growth defect of cut9ts cells at a restrictive temperature. cut9 encodes a component of the anaphase-promoting complex (APC) which is required for chromosome segregation at anaphase and moreover is defined as cyclin-specific ubiquitin ligase. Cdc13, a mitotic cyclin in fission yeast, was accumulated in the UbcP4-depleted cells. These results strongly suggested that UbcP4 is a ubiquitin-conjugating enzyme working in conjunction with APC and mediates the ubiquitin pathway for degradation of "sister chromatid holding protein(s)" at the onset of anaphase and possibly of mitotic cyclin at the exit of mitosis.
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