ArticleMolecular and cellular biology1997
Evidence that complex formation by Bas1p and Bas2p (Pho2p) unmasks the activation function of Bas1p in an adenine-repressible step of ADE gene transcription.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
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- A microarray-based genetic screen for yeast chronological aging factors.PLoS genetics · 2010Article
- Life in the midst of scarcity: adaptations to nutrient availability in Saccharomyces cerevisiae.Current genetics · 2010Review
- Cooperative regulation of ADE3 transcription by Gcn4p and Bas1p in Saccharomyces cerevisiae.Eukaryotic cell · 2009Article
- Metabolic intermediates selectively stimulate transcription factor interaction and modulate phosphate and purine pathways.Genes & development · 2009Article
- Candida glabrata PHO4 is necessary and sufficient for Pho2-independent transcription of phosphate starvation genes.Genetics · 2009Article
- New insights into the Saccharomyces cerevisiae fermentation switch: dynamic transcriptional response to anaerobicity and glucose-excess.BMC genomics · 2008Article
- Activation of the ADE genes requires the chromatin remodeling complexes SAGA and SWI/SNF.Eukaryotic cell · 2007Article
- Global analysis of the relationship between the binding of the Bas1p transcription factor and meiosis-specific double-strand DNA breaks in Saccharomyces cerevisiae.Molecular and cellular biology · 2006Article
- DNA-bound Bas1 recruits Pho2 to activate ADE genes in Saccharomyces cerevisiae.Eukaryotic cell · 2005Article
- Metabolic engineering of the purine pathway for riboflavin production in Ashbya gossypii.Applied and environmental microbiology · 2005Article
- Severe adenine starvation activates Ty1 transcription and retrotransposition in Saccharomyces cerevisiae.Molecular and cellular biology · 2005Article
- Yeast AMP pathway genes respond to adenine through regulated synthesis of a metabolic intermediate.Molecular and cellular biology · 2001Article
- The tRNA-binding moiety in GCN2 contains a dimerization domain that interacts with the kinase domain and is required for tRNA binding and kinase activation.The EMBO journal · 2001Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bas1p and Bas2p (Pho2p) are Myb-related and homeodomain DNA binding proteins, respectively, required for transcription of adenine biosynthetic genes in Saccharomyces cerevisiae. The repression of ADE genes in adenine-replete cells involves down-regulation of the functions of one or both of these activator proteins. A LexA-Bas2p fusion protein was found to activate transcription from a lexAop-lacZ reporter independently of both BAS1 function and the adenine levels in the medium. In contrast, a LexA-Bas1p fusion activated the lexAop reporter in a BAS2-dependent and adenine-regulated fashion. The DNA binding activity of Bas2p was not needed for its ability to support activation of the lexAop reporter by LexA-Bas1p, indicating that LexA-Bas1p recruits Bas2p to this promoter. The activation functions of both authentic Bas1p and LexA-Bas1p were stimulated under adenine-repressing conditions by overexpression of Bas2p, suggesting that complex formation by these proteins is inhibited in adenine-replete cells. Replacement of Asp-617 with Asn in Bas1p or LexA-Bas1p allowed either protein to activate transcription under repressing conditions in a manner fully dependent on Bas2p, suggesting that this mutation reduces the negative effect of adenine on complex formation by Bas1p and Bas2p. Deletions of N-terminal and C-terminal segments from the Bas1p moiety of LexA-Bas1p allowed high-level activation by the truncated proteins independently of Bas2p and adenine levels in the medium. From these results we propose that complex formation between Bas1p and Bas2p unmasks a latent activation function in Bas1p as a critical adenine-regulated step in transcription of the ADE genes.
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