ArticleMolecular and cellular biology1997
Rapamycin destabilizes interleukin-3 mRNA in autocrine tumor cells by a mechanism requiring an intact 3' untranslated region.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Inflammation-regulated mRNA stability and the progression of vascular inflammatory diseases.Clinical science (London, England : 1979) · 2017Review
- Destabilization of TNF-α mRNA by Rapamycin.Biomolecules & therapeutics · 2012Article
- The 2',3'-cAMP-adenosine pathway.American journal of physiology. Renal physiology · 2011Review
- 2',3'-cAMP, 3'-AMP, and 2'-AMP inhibit human aortic and coronary vascular smooth muscle cell proliferation via A2B receptors.American journal of physiology. Heart and circulatory physiology · 2011Article
- 2'-AMP and 3'-AMP inhibit proliferation of preglomerular vascular smooth muscle cells and glomerular mesangial cells via A2B receptors.The Journal of pharmacology and experimental therapeutics · 2011Article
- Identification and quantification of 2',3'-cAMP release by the kidney.The Journal of pharmacology and experimental therapeutics · 2009Article
- Signaling by target of rapamycin proteins in cell growth control.Microbiology and molecular biology reviews : MMBR · 2005Review
- The Herpesvirus saimiri small nuclear RNAs recruit AU-rich element-binding proteins but do not alter host AU-rich element-containing mRNA levels in virally transformed T cells.Molecular and cellular biology · 2004Article
- JAK/STAT, Raf/MEK/ERK, PI3K/Akt and BCR-ABL in cell cycle progression and leukemogenesis.Leukemia · 2004Review
- A GFP-based assay for rapid screening of compounds affecting ARE-dependent mRNA turnover.Nucleic acids research · 2004Article
- Article
- Elucidating TOR signaling and rapamycin action: lessons from Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2002Review
- The target of rapamycin signaling pathway regulates mRNA turnover in the yeast Saccharomyces cerevisiae.Molecular biology of the cell · 2001Article
- Parallel and independent regulation of interleukin-3 mRNA turnover by phosphatidylinositol 3-kinase and p38 mitogen-activated protein kinase.Molecular and cellular biology · 2001Article
- Article
- Calcium-induced stabilization of AU-rich short-lived mRNAs is a common default response.Gene expression · 1999Article
- Cyclosporin A promotes translational silencing of autocrine interleukin-3 via ribosome-associated deadenylation.Molecular and cellular biology · 1999Article
- c-jun N-terminal kinase is involved in AUUUA-mediated interleukin-3 mRNA turnover in mast cells.The EMBO journal · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We analyzed the effect of rapamycin on autocrine mast cell tumor lines with abnormally stable interleukin-3 (IL-3) transcripts due to a defect in mRNA degradation. Rapamycin inhibited IL-3 mRNA expression specifically, while transcripts of IL-4 and IL-6 were not affected. As indicated by the use of the transcriptional inhibitor actinomycin D or by reporter constructs, inhibition was posttranscriptional and resulted from destabilization of the mRNA. Transcripts from transgenes lacking the AU-rich 3' untranslated region were refractory to drug-induced degradation, suggesting that these 3' sequences contain the target of the rapamycin effect. Rapamycin did not promote IL-3 mRNA degradation in cells of a tumor variant lacking expression of FKBP12, the binding protein of rapamycin. Experiments with wortmannin indicated that rapamycin does not act via p70S6 kinase. FK-506, another ligand of FKBP12 affecting the phosphatase calcineurin, did not antagonize but shared the effect of rapamycin. Our data fit a model whereby both FKBP12 and calcineurin target an unknown regulator of IL-3 mRNA turnover.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.