ArticleMolecular and cellular biology1997
Overexpression of human release factor 1 alone has an antisuppressor effect in human cells.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 68 citations in OpenAlex.
- Variation in Release Factor Abundance Is Not Needed to Explain Trends in Bacterial Stop Codon Usage.Molecular biology and evolution · 2022Article
- Pharmacological approaches for targeting cystic fibrosis nonsense mutations.European journal of medicinal chemistry · 2020Review
- CTELS: A Cell-Free System for the Analysis of Translation Termination Rate.Biomolecules · 2020Article
- Eukaryotic translational termination efficiency is influenced by the 3' nucleotides within the ribosomal mRNA channel.Nucleic acids research · 2018Article
- Article
- The highly conserved codon following the slippery sequence supports -1 frameshift efficiency at the HIV-1 frameshift site.PloS one · 2015Article
- Efficient multisite unnatural amino acid incorporation in mammalian cells via optimized pyrrolysyl tRNA synthetase/tRNA expression and engineered eRF1.Journal of the American Chemical Society · 2014Article
- In vitro incorporation of nonnatural amino acids into protein using tRNA(Cys)-derived opal, ochre, and amber suppressor tRNAs.RNA (New York, N.Y.) · 2010Article
- RNAi-mediated knock-down of gene mN6A1 reduces cell proliferation and decreases protein translation.Molecular biology reports · 2009Article
- Human eukaryotic release factor 3a depletion causes cell cycle arrest at G1 phase through inhibition of the mTOR pathway.Molecular and cellular biology · 2007Article
- Cosuppression of eukaryotic release factor 1-1 in Arabidopsis affects cell elongation and radial cell division.Plant physiology · 2005Article
- Expression of mitochondrial release factor in relation to respiratory competence in yeast.Current genetics · 2005Article
- Involvement of human release factors eRF3a and eRF3b in translation termination and regulation of the termination complex formation.Molecular and cellular biology · 2005Article
- Genome-wide prediction of stop codon readthrough during translation in the yeast Saccharomyces cerevisiae.Nucleic acids research · 2004Article
- The effect of eukaryotic release factor depletion on translation termination in human cell lines.Nucleic acids research · 2004Article
- Stop codon suppression via inhibition of eRF1 expression.RNA (New York, N.Y.) · 2003Article
- Stop codon selection in eukaryotic translation termination: comparison of the discriminating potential between human and ciliate eRF1s.The EMBO journal · 2003Article
- Monitoring mis-acylated tRNA suppression efficiency in mammalian cells via EGFP fluorescence recovery.Nucleic acids research · 2002Article
- Inhibition of translation termination mediated by an interaction of eukaryotic release factor 1 with a nascent peptidyl-tRNA.Molecular and cellular biology · 2002Article
- The invariant uridine of stop codons contacts the conserved NIKSR loop of human eRF1 in the ribosome.The EMBO journal · 2002Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two eukaryotic proteins involved in translation termination have recently been characterized in in vitro experiments. Eukaryotic release factor 1 (eRF1) catalyzes the release of the polypeptide chain without any stop codon specificity. The GTP-binding protein eRF3 confers GTP dependence to the termination process and stimulates eRF1 activity. We used tRNA-mediated nonsense suppression at different stop codons in a cat reporter gene to analyze the polypeptide chain release factor activities of the human eRF1 and eRF3 proteins overexpressed in human cells. In a chloramphenicol acetyltransferase assay, we measured the competition between the suppressor tRNA and the human release factors when a stop codon was present in the ribosomal A site. Whatever the stop codon (UAA, UAG, or UGA) present in the cat open reading frame, the overexpression of human eRF1 alone markedly decreased translational readthrough by suppressor tRNA. Thus, like the procaryotic release factors RF1 and RF2 in Escherichia coli, eRF1 seems to have an intrinsic antisuppressor activity in human cells. Levels of antisuppression of overexpression of both eRF3 and eRF1 were almost the same as those of overexpression of eRF1 alone, suggesting that eRF1-eRF3 complex-mediated termination may be controlled by the expression level of eRF1. Surprisingly, when overexpressed alone, eRF3 had an inhibitory effect on cat gene expression. The results of cat mRNA stability studies suggest that eRF3 inhibits gene expression at the transcriptional level. This indicates that in vivo, eRF3 may perform other functions, including the stimulation of eRF1 activity.
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