ArticleMolecular and cellular biology1997
Role of the Ada adaptor complex in gene activation by the glucocorticoid receptor.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 73 citations in OpenAlex.
- Article
- Nucleosome distortion as a possible mechanism of transcription activation domain function.Epigenetics & chromatin · 2016Review
- Role of Nuclear Receptors in Central Nervous System Development and Associated Diseases.Journal of experimental neuroscience · 2015Review
- Determinants of the heightened activity of glucocorticoid receptor translational isoforms.Molecular endocrinology (Baltimore, Md.) · 2013Article
- Article
- Human ADA3 regulates RARalpha transcriptional activity through direct contact between LxxLL motifs and the receptor coactivator pocket.Nucleic acids research · 2010Article
- The SAGA subunit Ada2 functions in transcriptional silencing.Molecular and cellular biology · 2009Article
- Mechanisms underlying the control of progesterone receptor transcriptional activity by SUMOylation.The Journal of biological chemistry · 2009Article
- Differential regulation of the transcriptional activity of the glucocorticoid receptor through site-specific phosphorylation.Biologics : targets & therapy · 2008Article
- Functional conservation of the glutamine-rich domains of yeast Gal11 and human SRC-1 in the transactivation of glucocorticoid receptor Tau 1 in Saccharomyces cerevisiae.Molecular and cellular biology · 2008Article
- Structure and function of steroid receptor AF1 transactivation domains: induction of active conformations.The Biochemical journal · 2005Review
- TATA box binding protein induces structure in the recombinant glucocorticoid receptor AF1 domain.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Human papillomavirus oncoprotein E6 inactivates the transcriptional coactivator human ADA3.Molecular and cellular biology · 2002Article
- The role of coactivators and corepressors in the biology and mechanism of action of steroid hormone receptors.Journal of mammary gland biology and neoplasia · 2000Review
- Recruitment of the SWI-SNF chromatin remodeling complex as a mechanism of gene activation by the glucocorticoid receptor tau1 activation domain.Molecular and cellular biology · 2000Article
- A ligand binding domain mutation in the mouse glucocorticoid receptor functionally links chromatin remodeling and transcription initiation.Molecular and cellular biology · 1999Article
- Histone acetyltransferase complexes can mediate transcriptional activation by the major glucocorticoid receptor activation domain.Molecular and cellular biology · 1999Article
- Role of the essential yeast protein PSU1 in p6anscriptional enhancement by the ligand-dependent activation function AF-2 of nuclear receptors.The EMBO journal · 1999Article
- Activation domain-specific and general transcription stimulation by native histone acetyltransferase complexes.Molecular and cellular biology · 1999Article
- Identification of a novel RING finger protein as a coregulator in steroid receptor-mediated gene transcription.Molecular and cellular biology · 1998Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have shown that the Ada adaptor complex is important for the gene activation capacity of the glucocorticoid receptor in yeast. The recently isolated human Ada2 protein also increases the potency of the receptor protein in mammalian cells. The Ada pathway is of key significance for the tau1 core transactivation domain (tau1c) of the receptor, which requires Ada for activity in vivo and in vitro. Ada2 can be precipitated from nuclear extracts by a glutathione S-transferase-tau1 fusion protein coupled to agarose beads, and a direct interaction between Ada2 and tau1c can be shown by using purified proteins. This interaction is strongly reduced by a mutation in tau1c that reduces transactivation activity. Mutations affecting the Ada complex do not reverse transcriptional squelching by the tau1 domain, as they do for the VP16 transactivation domain, and thus these powerful acidic activators differ in at least some important aspects of gene activation. Mutations that reduce the activity of the tau1c domain in wild-type yeast strains cause similar reductions in ada mutants that contain little or no Ada activity. Thus, gene activation mechanisms, in addition to the Ada pathway, are involved in the activity of the tau1c domain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.