ArticleNucleic acids research1997
Direct identification of differentially expressed genes by cycle sequencing and cycle labelling using the differential display PCR primers.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Genomewide production of multipurpose alleles for the functional analysis of the mouse genome.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- CLN1 and its repression by Xbp1 are important for efficient sporulation in budding yeast.Molecular and cellular biology · 2000Article
- Article
- Multiple isoform recovery (MIR)-PCR: a simple method for the isolation of related mRNA isoforms.Nucleic acids research · 1998Article
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Authors and funding
3 authors.
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Abstract
Differential display PCR (DD-PCR) is an mRNA fingerprinting technique to identify differentially expressed genes by comparative display of arbitrarily amplified cDNA subsets. This attractively simple screening method was, however, followed by a labour intensive multistep identification procedure for DD-PCR products. In this report we demonstrate for the mouse mast cell protease 2 (MMCP-2) and the cytotoxic T-lymphocyte associated gene transcript CTLA-1 a streamlined approach by (i) direct cycle sequencing with the upstream differential display (DD) primer, followed by (ii) the PCR based generation of an antisense northern probe with the downstream anchor primer.
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