ArticleNucleic acids research1997
Differential in vivo activation of the class II and class III snRNA genes by the POU-specific domain of Oct-1.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- CAPTURE of the Human U2 snRNA Genes Expands the Repertoire of Associated Factors.Biomolecules · 2022Article
- Regulation of expression of human RNA polymerase II-transcribed snRNA genes.Open biology · 2017Review
- Lentiviral vectors encoding tetracycline-dependent repressors and transactivators for reversible knockdown of gene expression: a comparative study.BMC biotechnology · 2007Article
- BRCA1 cooperates with NUFIP and P-TEFb to activate transcription by RNA polymerase II.Oncogene · 2004Article
- The C-terminal domain of pol II and a DRB-sensitive kinase are required for 3' processing of U2 snRNA.The EMBO journal · 2003Article
- Activator recruitment by the general transcription machinery: X-ray structural analysis of the Oct-1 POU domain/human U1 octamer/SNAP190 peptide ternary complex.Genes & development · 2002Article
- Intergenic transcription in the human beta-globin gene cluster.Molecular and cellular biology · 2001Article
- Transcription of the human U2 snRNA genes continues beyond the 3' box in vivo.The EMBO journal · 1999Article
- The activation function 2 domain of hepatic nuclear factor 4 is regulated by a short C-terminal proline-rich repressor domain.Nucleic acids research · 1998Article
- A tandem array of minimal U1 small nuclear RNA genes is sufficient to generate a new adenovirus type 12-inducible chromosome fragile site.Journal of virology · 1998Article
- Two distinct domains in Staf to selectively activate small nuclear RNA-type and mRNA promoters.Molecular and cellular biology · 1998Article
- The microsatellite sequence (CT)n x (GA)n promotes stable chromosomal integration of large tandem arrays of functional human U2 small nuclear RNA genes.Molecular and cellular biology · 1998Article
- The distal elements, OCT and SPH, stimulate the formation of preinitiation complexes on a human U6 snRNA gene promoter in vitro.Nucleic acids research · 1998Article
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Authors and funding
1 author.
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Abstract
Many snRNA genes contain binding sites for the ubiquitous transcription factor Oct-1. In vitro studies have shown that this factor potentiates binding of an essential transcription factor (PTF) to the proximal sequence element (PSE) of snRNA genes, and activates transcription. Using Gal4 fusion proteins, I show here that the POU-specific region of the DNA-binding domain of Oct-1 is sufficient both to potentiate PTF binding in vitro and to transactivate pol II- and pol III-dependent snRNA genes in vivo . A single amino acid change in this domain abrogates both activation and interaction with PTF. The N- and C-terminal regions of Oct-1 also activate transcription of both classes of snRNA genes. Wild-type levels of Pol II-dependent U2 expression require all activation domains, whereas efficient activation of the pol III-dependent 7SK and U6 genes is effected by the POU-specific domain alone. These results indicate that contacts between PTF and amino acids in the POU-specific domain of Oct-1 are critical for efficient transactivation of snRNA genes in vivo. The POU-specific domain of Oct-2A also activates these genes, but the N- and C-terminal domains are relatively inactive.
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