ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1997
Calcium entry through cyclic nucleotide-gated channels in individual cilia of olfactory receptor cells: spatiotemporal dynamics.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed, 149 citations in OpenAlex.
- Contribution of TRPC3-mediated CaPflugers Archiv : European journal of physiology · 2023Article
- Shedding light on human olfaction: Electrophysiological recordings from sensory neurons in acute slices of olfactory epithelium.iScience · 2023Article
- Segregation of Ca2+ signaling in olfactory signal transduction.The Journal of general physiology · 2023Article
- The β-cell primary cilium is an autonomous Ca2+ compartment for paracrine GABA signaling.The Journal of cell biology · 2023Article
- Transduction and Adaptation Mechanisms in the Cilium or Microvilli of Photoreceptors and Olfactory Receptors From Insects to Humans.Frontiers in cellular neuroscience · 2021Review
- Stimulus Driven Functional Transformations in the Early Olfactory System.Frontiers in cellular neuroscience · 2021Article
- The cyclic AMP signaling pathway in the rodent main olfactory system.Cell and tissue research · 2021Review
- Maturation of the Olfactory Sensory Neuron and Its Cilia.Chemical senses · 2020Review
- Integrating Bioelectrical Currents and CaBioelectricity · 2020Review
- Second messenger molecules have a limited spread in olfactory cilia.The Journal of general physiology · 2018Article
- Spontaneously active NaV1.5 sodium channels may underlie odor sensitivity.Journal of neurophysiology · 2016Article
- From damage response to action potentials: early evolution of neural and contractile modules in stem eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Cyclic GMP and Cilia Motility.Cells · 2015Review
- Electrical Signaling in Motile and Primary Cilia.Bioscience · 2014Article
- New perspectives in cyclic nucleotide-mediated functions in the CNS: the emerging role of cyclic nucleotide-gated (CNG) channels.Pflugers Archiv : European journal of physiology · 2014Review
- Increases in intracellular calcium via activation of potentially multiple phospholipase C isozymes in mouse olfactory neurons.Frontiers in cellular neuroscience · 2014Article
- Mechanisms underlying odorant-induced and spontaneous calcium signals in olfactory receptor neurons of spiny lobsters, Panulirus argus.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2014Article
- Functional evidence of multidrug resistance transporters (MDR) in rodent olfactory epithelium.PloS one · 2012Article
- Review
- Cyclic nucleotide-gated channels contribute to thromboxane A2-induced contraction of rat small mesenteric arteries.PloS one · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 2 countries.
Funding
Abstract
Transient elevations of intracellular Ca2+ play an important role in regulating the sensitivity of olfactory transduction, but such elevations have not been demonstrated in the olfactory cilia, which are the site of primary odor transduction. To begin to understand Ca2+ signaling in olfactory cilia, we used high-resolution imaging techniques to study the Ca2+ transients that occur in salamander olfactory receptor neurons (ORNs) as a result of cyclic nucleotide-gated (CNG) channel activation. To visualize ciliary Ca2+ signals, we loaded ORNs with the Ca2+ indicator dye Fluo-3 AM and measured fluorescence with a laser scanning confocal microscope. Application of the phosphodiesterase inhibitor IBMX increased fluorescence in the cilia and other neuronal compartments; the ciliary signal occurred first and was more transient. This signal could be abolished by lowering external Ca2+ or by applying LY83583, a potent blocker of CNG channels, indicating that Ca2+ entry through CNG channels was the primary source of fluorescence increases. Direct activation of CNG channels with low levels of 8-Br-cGMP (1 microM) led to tonic Ca2+ signals that were restricted locally to the cilia and the dendritic knob. Elevated external K+, which depolarizes cell membranes, increased fluorescence signals in the cell body and dendrite but failed to increase ciliary Ca2+ fluorescence. The results demonstrate the existence and spatiotemporal properties of Ca2+ transients in individual olfactory cilia and implicate CNG channels as a major pathway for Ca2+ entry into ORN cilia during odor transduction.
Indexed as
Identifiers
9151731PMC6573532W1895908349What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.