ArticleThe Journal of experimental medicine1997
Kinetics and extent of T cell activation as measured with the calcium signal.
Article in The Journal of experimental medicine, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
- Proofreading and single-molecule sensitivity in T cell receptor signaling by condensate nucleation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
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- Time-resolved role of P2X4 and P2X7 during CD8Frontiers in immunology · 2024Article
- Sustained store-operated calcium entry utilizing activated chromatin state leads to instability in iTregs.eLife · 2023Article
- Monomeric agonist peptide/MHCII complexes activate T-cells in an autonomous fashion.EMBO reports · 2023Article
- T cell microvilli simulations show operation near packing limit and impact on antigen recognition.Biophysical journal · 2022Article
- Signal strength controls the rate of polarization within CTLs during killing.The Journal of cell biology · 2021Article
- Temporal analysis of T-cell receptor-imposed forces via quantitative single molecule FRET measurements.Nature communications · 2021Article
- Review
- Article
- Soft Polydimethylsiloxane-Supported Lipid Bilayers for Studying T Cell Interactions.Biophysical journal · 2021Article
- Heterogeneous T cell motility behaviors emerge from a coupling between speed and turning in vivo.eLife · 2020Article
- Article
- Regulation of T Helper Cell Fate by TCR Signal Strength.Frontiers in immunology · 2020Review
- Dynamic Mitochondrial Migratory Features Associated with Calcium Responses during T Cell Antigen Recognition.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- A correlative and quantitative imaging approach enabling characterization of primary cell-cell communication: Case of human CD4Scientific reports · 2018Article
- ROCK regulates the intermittent mode of interstitial T cell migration in inflamed lungs.Nature communications · 2017Article
- CalQuoScientific reports · 2017Article
- Evaluating frequency and quality of pathogen-specific T cells.Nature communications · 2016Article
- T cell migration, search strategies and mechanisms.Nature reviews. Immunology · 2016Review
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6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have characterized the calcium response of a peptide-major histocompatibility complex (MHC)-specific CD4(+) T lymphocyte line at the single cell level using a variety of ligands, alone and in combination. We are able to distinguish four general patterns of intracellular calcium elevation, with only the most robust correlating with T cell proliferation. Whereas all three antagonist peptides tested reduce the calcium response to an agonist ligand, two give very different calcium release patterns and the third gives none at all, arguing that (a) antagonism does not require calcium release and (b) it involves interactions that are more T cell receptor proximal. We have also measured the time between the first T cell-antigen-presenting cell contact and the onset of the calcium signal. The duration of this delay correlates with the strength of the stimulus, with stronger stimuli giving a more rapid response. The dose dependence of this delay suggests that the rate-limiting step in triggering the calcium response is not the clustering of peptide-MHC complexes on the cell surface but more likely involves the accumulation of some intracellular molecule or complex with a half-life of a few minutes.
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