ArticleThe Journal of cell biology1997
Connexin46 is retained as monomers in a trans-Golgi compartment of osteoblastic cells.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
53 citing papers in PubMed, 159 citations in OpenAlex.
- Skeletal abnormalities caused by a Connexin43Bone research · 2025Article
- Connexin 43 mediated collective cell migration is independent of Golgi orientation.Biology open · 2023Article
- Article
- Functional Roles of Connexins and Gap Junctions in Osteo-Chondral Cellular Components.International journal of molecular sciences · 2023Review
- Connexin43 in Musculoskeletal System: New Targets for Development and Disease Progression.Aging and disease · 2022Article
- Extracellular Cysteines Are Critical to Form Functional Cx46 Hemichannels.International journal of molecular sciences · 2022Article
- Role of the Connexin C-terminus in skin pattern formation of Zebrafish.BBA advances · 2021Article
- Connexin-46 Contained in Extracellular Vesicles Enhance Malignancy Features in Breast Cancer Cells.Biomolecules · 2020Article
- Maturation-driven transport and AP-1-dependent recycling of a secretory cargo in the Golgi.The Journal of cell biology · 2019Article
- Contribution of Connexin Hemichannels to the Decreases in Cell Viability Induced by Linoleic Acid in the Human Lens Epithelial Cells (HLE-B3).Frontiers in physiology · 2019Article
- Concatenation of Human Connexin26 (hCx26) and Human Connexin46 (hCx46) for the Analysis of Heteromeric Gap Junction Hemichannels and Heterotypic Gap Junction Channels.International journal of molecular sciences · 2018Article
- Connexins: Synthesis, Post-Translational Modifications, and Trafficking in Health and Disease.International journal of molecular sciences · 2018Review
- Joint diseases: from connexins to gap junctions.Nature reviews. Rheumatology · 2017Review
- The connexin 46 mutant (V44M) impairs gap junction function causing congenital cataract.Journal of genetics · 2017Article
- Connexins in Cardiovascular and Neurovascular Health and Disease: Pharmacological Implications.Pharmacological reviews · 2017Review
- microRNAs and connexins in bone: interaction and mechanisms of delivery.Current molecular biology reports · 2017Article
- Connexin43 and the Intercellular Signaling Network Regulating Skeletal Remodeling.Current osteoporosis reports · 2017Review
- Cardiac Cx43, Cx40 and Cx45 co-assembling: involvement of connexins epitopes in formation of hemichannels and Gap junction channels.BMC cell biology · 2017Review
- Distinctive actions of connexin 46 and connexin 50 in anterior pituitary folliculostellate cells.PloS one · 2017Article
- Role of connexins and pannexins during ontogeny, regeneration, and pathologies of bone.BMC cell biology · 2016Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Connexins are gap junction proteins that form aqueous channels to interconnect adjacent cells. Rat osteoblasts express connexin43 (Cx43), which forms functional gap junctions at the cell surface. We have found that ROS 17/2.8 osteosarcoma cells, UMR 106-01 osteosarcoma cells, and primary rat calvarial osteoblastic cells also express another gap junction protein, Cx46. Cx46 is a major component of plasma membrane gap junctions in lens. In contrast, Cx46 expressed by osteoblastic cells was predominantly localized to an intracellular perinuclear compartment, which appeared to be an aspect of the TGN as determined by immunofluorescence colocalization. Hela cells transfected with rat Cx46 cDNA (Hela/Cx46) assembled Cx46 into functional gap junction channels at the cell surface. Both rat lens and Hela/Cx46 cells expressed 53-kD (nonphosphorylated) and 68-kD (phosphorylated) forms of Cx46; however, only the 53-kD form was produced by osteoblasts. To examine connexin assembly, monomers were resolved from oligomers by sucrose gradient velocity sedimentation analysis of 1% Triton X-100-solubilized extracts. While Cx43 was assembled into multimeric complexes, ROS cells contained only the monomer form of Cx46. In contrast, Cx46 expressed by rat lens and Hela/Cx46 cells was assembled into multimers. These studies suggest that assembly and cell surface expression of two closely related connexins were differentially regulated in the same cell. Furthermore, oligomerization may be required for connexin transport from the TGN to the cell surface.
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Registered trials
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