ArticleThe Journal of cell biology1997
A nuclear export signal in Kap95p is required for both recycling the import factor and interaction with the nucleoporin GLFG repeat regions of Nup116p and Nup100p.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 84 citations in OpenAlex.
- Septin-associated proteins Aim44 and Nis1 traffic between the bud neck and the nucleus in the yeast Saccharomyces cerevisiae.Cytoskeleton (Hoboken, N.J.) · 2019Article
- Simple rules for passive diffusion through the nuclear pore complex.The Journal of cell biology · 2016Article
- Size-dependent leak of soluble and membrane proteins through the yeast nuclear pore complex.Molecular biology of the cell · 2015Article
- A subset of FG-nucleoporins is necessary for efficient Msn5-mediated nuclear protein export.Biochimica et biophysica acta · 2013Article
- Facilitated transport and diffusion take distinct spatial routes through the nuclear pore complex.Journal of cell science · 2010Article
- Controlling subcellular delivery to optimize therapeutic effect.Therapeutic delivery · 2010Review
- The Ty1 integrase protein can exploit the classical nuclear protein import machinery for entry into the nucleus.Nucleic acids research · 2008Article
- The karyopherin Kap95 regulates nuclear pore complex assembly into intact nuclear envelopes in vivo.Molecular biology of the cell · 2007Article
- Controlling protein compartmentalization to overcome disease.Pharmaceutical research · 2007Review
- Yeast phosphatidylinositol 4-kinase, Pik1, has essential roles at the Golgi and in the nucleus.The Journal of cell biology · 2005Article
- Nuclear envelope irregularity is induced by RET/PTC during interphase.The American journal of pathology · 2003Article
- Transport into and out of the nucleus.Microbiology and molecular biology reviews : MMBR · 2001Review
- Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export.The Journal of cell biology · 2001Article
- Calcineurin-dependent nuclear import of the transcription factor Crz1p requires Nmd5p.The Journal of cell biology · 2001Article
- The yeast hnRNP-Like proteins Yra1p and Yra2p participate in mRNA export through interaction with Mex67p.Molecular and cellular biology · 2001Article
- Purification of the vertebrate nuclear pore complex by biochemical criteria.Traffic (Copenhagen, Denmark) · 2000Article
- Nup2p, a yeast nucleoporin, functions in bidirectional transport of importin alpha.Molecular and cellular biology · 2000Article
- Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export.The Journal of cell biology · 2000Article
- Assembly and preferential localization of Nup116p on the cytoplasmic face of the nuclear pore complex by interaction with Nup82p.Molecular and cellular biology · 2000Article
- Interactions between a nuclear transporter and a subset of nuclear pore complex proteins depend on Ran GTPase.Molecular and cellular biology · 1999Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
During nuclear import, cytosolic transport factors move through the nuclear pore complex (NPC) to the nuclear compartment. Kap95p is required during import for docking the nuclear localization signal-receptor and ligand to the NPC. Recycling of this factor back to the cytoplasm is necessary for continued rounds of import; however, the mechanism for Kap95p recycling is unknown. We have determined that recycling of Kap95p requires a nuclear export signal (NES). A region containing the NES in Kap95p was sufficient to mediate active nuclear export in a microinjection assay. Moreover, the NES was necessary for function. Mutation of the NES in Kap95p resulted in a temperaturesensitive import mutant, and immunofluorescence microscopy experiments showed that the mutated Kap95p was not recycled but instead localized in the nucleus and at the nuclear envelope. Srp1p, the yeast nuclear localization signal-receptor, also accumulated in the nuclei of the arrested kap95 mutant cells. Wild-type and NES-mutated Kap95p both bound Gsp1p (the yeast Ran/TC4 homologue), Srp1p, and the FXFG repeat region of the nucleoporin Nup1p. In contrast, the NES mutation abolished Kap95p interaction with the GLFG repeat regions from the nucleoporins Nup116p and Nup100p. In vivo interaction was demonstrated by isolation of Kap95p from yeast nuclear lysates in either protein A-tagged Nup116p or protein A-tagged Nup100p complexes. The protein A-tagged Nup116p complex also specifically contained Gle2p. These results support a model in which a step in the recycling of Kap95p is mediated by interaction of an NES with GLFG regions. Analysis of genetic interactions suggests Nup116p has a primary role in Kap95p recycling, with Nup100p compensating in the absence of Nup116p. This finding highlights an important role for a subfamily of GLFG nucleoporins in nuclear export processes.
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