ArticleThe Journal of cell biology1997
An unexpected localization of basonuclin in the centrosome, mitochondria, and acrosome of developing spermatids.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 58 citations in OpenAlex.
- Ploidy and Implantation Potential: Non-Invasive Small Non-Coding RNA-Based Health Assessment of Day 5 and 6 Blastocysts.International journal of molecular sciences · 2025Article
- Toward clinical exomes in diagnostics and management of male infertility.American journal of human genetics · 2024Article
- Truncating PICK1 Variant Identified in Azoospermia Affected Mitochondrial Dysfunction in Knockout Mice.Current medical science · 2023Article
- Is transcription in sperm stationary or dynamic?The Journal of reproduction and development · 2017Review
- BNC1 is required for maintaining mouse spermatogenesis.Genesis (New York, N.Y. : 2000) · 2012Article
- Human balanced translocation and mouse gene inactivation implicate Basonuclin 2 in distal urethral development.European journal of human genetics : EJHG · 2011Article
- The transcriptional repressor Kaiso localizes at the mitotic spindle and is a constituent of the pericentriolar material.PloS one · 2010Article
- Basonuclin-2 requirements for zebrafish adult pigment pattern development and female fertility.PLoS genetics · 2009Article
- Basonuclin 2 has a function in the multiplication of embryonic craniofacial mesenchymal cells and is orthologous to disco proteins.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Basonuclins 1 and 2, whose genes share a common origin, are proteins with widely different properties and functions.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Mouse SYCP2 is required for synaptonemal complex assembly and chromosomal synapsis during male meiosis.The Journal of cell biology · 2006Article
- Re-epithelialisation and the possible involvement of the transcription factor, basonuclin.International wound journal · 2004Review
- Basonuclin 2: an extremely conserved homolog of the zinc finger protein basonuclin.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Marker succession during the development of keratinocytes from cultured human embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Article
- Basonuclin, a zinc finger protein of keratinocytes and reproductive germ cells, binds to the rRNA gene promoter.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- A novel 95-kD protein is located in a linker between cytoplasmic microtubules and basal bodies in a green flagellate and forms striated filaments in vitro.The Journal of cell biology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Basonuclin is a zinc finger protein that was thought to be restricted to keratinocytes of stratified squamous epithelia. In epidermis, basonuclin is associated with the nuclei of mitotically active basal cells but not in terminally differentiating keratinocytes. We report here the isolation of a novel form of basonuclin, which we show is also expressed in stratified epithelia. Most unexpectedly, we find both forms in testis, where a surprising localization pattern was uncovered. While basonuclin RNA expression occurs in mitotically active germ cells, protein was not detected until the meiotic stage, where basonuclin localized to the appendage of the distal centriole of spermatocytes and spermatids. Near the end of spermiogenesis, basonuclin also accumulated in the acrosome and mitochondrial sheath surrounding the flagellum. Intriguingly, a perfect six-amino acid residue mitochondrial targeting sequence (Komiya, T., N. Hachiya, M. Sakaguchi, T. Omura, and K. Mihara. 1994. J. Biol. Chem. 269:30893-30897; Shore, G.C., H.M. McBride, D.G. Millar, N.A. Steenaart, and M. Nguyen. 1995. Eur. J. Biochem. 227: 9-18; McBride, H.M., I.S. Goping, and G.C. Shore. 1996. J. Cell. Biol. 134:307-313) is present in basonuclin 1a but not in the 1b form. Moreover, three distinct affinity-purified peptide antibodies gave this unusual pattern of basonuclin antibody staining, which was confirmed by cell fractionation studies. Our findings suggest a unique role for basonuclin in centrosomes within the developing spermatid, and a role for one of the protein forms in germ cell mitochondrial function. Its localization with the acrosome suggests that it may also perform a special function during or shortly after fertilization.
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