ArticleThe Journal of cell biology1997
Subnuclear trafficking of glucocorticoid receptors in vitro: chromatin recycling and nuclear export.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 104 citations in OpenAlex.
- Pharmacokinetics/pharmacodynamics of glucocorticoids: modeling the glucocorticoid receptor dynamics and dose/response of commonly prescribed glucocorticoids.ADMET & DMPK · 2024Article
- Glucocorticoids, their uses, sexual dimorphisms, and diseases: new concepts, mechanisms, and discoveries.Physiological reviews · 2024Review
- Biological Actions of the Hsp90-binding Immunophilins FKBP51 and FKBP52Biomolecules · 2019Review
- Ultradian glucocorticoid exposure directs gene-dependent and tissue-specific mRNA expression patterns in vivo.Molecular and cellular endocrinology · 2017Article
- Role of Ran-regulated nuclear-cytoplasmic trafficking of pVHL in the regulation of microtubular stability-mediated HIF-1α in hypoxic cardiomyocytes.Scientific reports · 2015Article
- Altered transcription factor trafficking in oxidatively-stressed neuronal cells.Biochimica et biophysica acta · 2012Article
- Complex dynamics of transcription regulation.Biochimica et biophysica acta · 2012Review
- The HSP90 molecular chaperone cycle regulates cyclical transcriptional dynamics of the glucocorticoid receptor and its coregulatory molecules CBP/p300 during ultradian ligand treatment.Molecular endocrinology (Baltimore, Md.) · 2011Article
- The hsp90-FKBP52 complex links the mineralocorticoid receptor to motor proteins and persists bound to the receptor in early nuclear events.Molecular and cellular biology · 2010Article
- Crosstalk in inflammation: the interplay of glucocorticoid receptor-based mechanisms and kinases and phosphatases.Endocrine reviews · 2009Review
- Activation of the DNA-dependent protein kinase stimulates nuclear export of the androgen receptor in vitro.The Journal of biological chemistry · 2008Article
- Geldanamycin, an inhibitor of Hsp90, blocks cytoplasmic retention of progesterone receptors and glucocorticoid receptors via their respective ligand binding domains.The AAPS journal · 2006Article
- Import and export of nuclear proteins: focus on the nucleocytoplasmic movements of two different species of mammalian estrogen receptor.Molecular and cellular biochemistry · 2004Review
- Molecular chaperones function as steroid receptor nuclear mobility factors.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Nuclear export in plants. Use of geminivirus movement proteins for a cell-based export assay.The Plant cell · 1999Article
- Discrimination between NL1- and NL2-mediated nuclear localization of the glucocorticoid receptor.Molecular and cellular biology · 1999Article
- Reconstitution of HIV-1 rev nuclear export: independent requirements for nuclear import and export.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Nucleocytoplasmic shuttling factors including Ran and CRM1 mediate nuclear export of NFAT In vitro.The Journal of cell biology · 1998Article
- Glucocorticoid receptor: implications for rheumatic diseases.Clinical and experimental rheumatologyReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have used digitonin-permeabilized cells to examine in vitro nuclear export of glucocorticoid receptors (GRs). In situ biochemical extractions in this system revealed a distinct subnuclear compartment, which collects GRs that have been released from chromatin and serves as a nuclear export staging area. Unliganded nuclear GRs within this compartment are not restricted in their subnuclear trafficking as they have the capacity to recycle to chromatin upon rebinding hormone. Thus, GRs that release from chromatin do not require transit through the cytoplasm to regain functionality. In addition, chromatin-released receptors export from nuclei of permeabilized cells in an ATP- and cytosol-independent process that is stimulated by sodium molybdate, other group VI-A transition metal oxyanions, and some tyrosine phosphatase inhibitors. The stimulation of in vitro nuclear export by these compounds is not unique to GR, but is restricted to other proteins such as the 70- and 90-kD heat shock proteins, hsp70 and hsp90, respectively, and heterogeneous nuclear RNP (hnRNP) A1. Under analogous conditions, the 56-kD heat shock protein, hsp56, and hnRNP C do not export from nuclei of permeabilized cells. If tyrosine kinase inhibitors genistein and tyrphostin AG126 are included to prevent increased tyrosine phosphorylation, in vitro nuclear export of GR is inhibited. Thus, our results are consistent with the involvement of a phosphotyrosine system in the general regulation of nuclear protein export, even for proteins such as GR and hnRNP A1 that use distinct nuclear export pathways.
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