ArticleGenome research1997
A transposon-based strategy for sequencing repetitive DNA in eukaryotic genomes.
Article in Genome research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Detecting foldback artifacts in long-reads.BMC genomics · 2026Article
- Detecting Foldback Artifacts in Long-reads.bioRxiv : the preprint server for biology · 2025Article
- SPTEdb: a database for transposable elements in salicaceous plants.Database : the journal of biological databases and curation · 2018Article
- Article
- Harnessing mobile genetic elements to explore gene regulation.Mobile genetic elements · 2014Review
- Primary analysis of repeat elements of the Asian seabass (Lates calcarifer) transcriptome and genome.Frontiers in genetics · 2014Article
- Large-scale sequencing based on full-length-enriched cDNA libraries in pigs: contribution to annotation of the pig genome draft sequence.BMC genomics · 2012Article
- A guided tour of large genome size in animals: what we know and where we are heading.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Review
- Novel sequencing strategy for repetitive DNA in a Drosophila BAC clone reveals that the centromeric region of the Y chromosome evolved from a telomere.Nucleic acids research · 2009Article
- Identification of transposable elements using multiple alignments of related genomes.Genome research · 2006Article
- The small chromosomes of Trypanosoma brucei involved in antigenic variation are constructed around repetitive palindromes.Genome research · 2004Article
- Construction and utility of 10-kb libraries for efficient clone-gap closure for rice genome sequencing.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2003Article
- Short inverted repeats initiate gene amplification through the formation of a large DNA palindrome in mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- RNA hairpins in noncoding regions of human brain and Caenorhabditis elegans mRNA are edited by adenosine deaminases that act on RNA.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Systematic sequencing of cDNA clones using the transposon Tn5.Nucleic acids research · 2002Article
- An efficient strategy for large-scale high-throughput transposon-mediated sequencing of cDNA clones.Nucleic acids research · 2002Article
- The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective.Genome biology · 2002Article
- Transposons but not retrotransposons are located preferentially in regions of high recombination rate in Caenorhabditis elegans.Genetics · 2000Article
- Long palindromes formed in Streptomyces by nonrecombinational intra-strand annealing.Genes & development · 2000Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Repetitive DNA is a significant component of eukaryotic genomes. We have developed a strategy to efficiently and accurately sequence repetitive DNA in the nematode Caenorhabditis elegans using integrated artificial transposons and automated fluorescent sequencing. Mapping and assembly tools represent important components of this strategy and facilitate sequence assembly in complex regions. We have applied the strategy to several cosmid assembly gaps resulting from repetitive DNA and have accurately recovered the sequences of these regions. Analysis of these regions revealed six novel transposon-like repetitive elements, IR-1, IR-2, IR-3, IR-4, IR-5, and TR-1. Each of these elements represents a middle-repetitive DNA family in C. elegans containing at least 3-140 copies per genome. Copies of IR-1, IR-2, IR-4, and IR-5 are located on all (or most) of the six nematode chromosomes, whereas IR-3 is predominantly located on chromosome X. These elements are almost exclusively interspersed between predicted genes or within the predicted introns of these genes, with the exception of a single IR-5 element, which is located within a predicted exon. IR-1, IR-2, and IR-3 are flanked by short sequence duplications resembling the target site duplications of transposons. We have established a website database (http:(/)/www.welch.jhu.edu/approximately devine/RepDNAdb.html) to track and cross-reference these transposon-like repetitive elements that contains detailed information on individual element copies and provides links to appropriate GenBank records. This set of tools may be used to sequence, track, and study repetitive DNA in model organisms and humans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.