ArticleGenome research1997
Sequence mapping by electronic PCR
Article in Genome research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 174 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
174 citing papers in PubMed, 403 citations in OpenAlex.
- Distinction BetweenJournal of fungi (Basel, Switzerland) · 2025Article
- Comprehensive SSR study of 14 Zingiberaceae species based on microsatellite capture sequencing (MiCAPs).Frontiers in plant science · 2025Article
- Application of an Anchor Mapping of Alien Chromosome (AMAC) Fragment Localization Method in the Identification of Radish Chromosome Segments in the Progeny of Rape-Radish Interspecific Hybrids.International journal of molecular sciences · 2024Article
- SlJMJ14, identified via QTL‑seq and fine mapping, controls flowering time in tomatoes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Genome-wide development of intra- and inter-specific transferable SSR markers and construction of a dynamic web resource for yam molecular breeding: Y2MD.The plant genome · 2024Article
- Development of Genome-Wide Intron Length Polymorphism (ILP) Markers in Tea Plant (International journal of molecular sciences · 2024Article
- SoyMD: a platform combining multi-omics data with various tools for soybean research and breeding.Nucleic acids research · 2024Article
- What are microsatellites and how to choose the best tool: a user-friendly review of SSR and 74 SSR mining tools.Frontiers in genetics · 2024Review
- Fast and Accurate Multiplex Identification and Quantification of Seven Genetically Modified Soybean Lines Using Six-Color Digital PCR.Foods (Basel, Switzerland) · 2023Article
- Comprehensive genome-wide identification and transferability of chromosome-specific highly variable microsatellite markers from citrus species.Scientific reports · 2023Article
- New insight into the genetic basis of oil content based on noninvasive three-dimensional phenotyping and tissue-specific transcriptome in Brassica napus.Biotechnology for biofuels and bioproducts · 2023Article
- In Silico Integrated Analysis of Genomic, Transcriptomic, and Proteomic Data Reveals QTL-Specific Genes for Bacterial Canker Resistance in Tomato (Current issues in molecular biology · 2023Article
- Resistance of QYm.nau-2D to wheat yellow mosaic virus was derived from an alien introgression into common wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Article
- R gene triplication confers European fodder turnip with improved clubroot resistance.Plant biotechnology journal · 2022Article
- Identification and validation of SSR markers for3 Biotech · 2022Article
- Genome Assembly and Analysis of the Flavonoid and Phenylpropanoid Biosynthetic Pathways in Fingerroot Ginger (International journal of molecular sciences · 2022Article
- Discrimination and Detection of Erwinia amylovora and Erwinia pyrifoliae with a Single Primer Set.The plant pathology journal · 2022Article
- SSR2Marker: an integrated pipeline for identification of SSR markers within any two given genome-scale sequences.Molecular horticulture · 2022Article
- Construction of a Quantitative Genomic Map, Identification and Expression Analysis of Candidate Genes for Agronomic and Disease-Related Traits inFrontiers in plant science · 2022Article
114 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The highly specific and sensitive PCR provides the basis for sequence-tagged sites (STSs), unique landmarks that have been used widely in the construction of genetic and physical maps of the human genome. Electronic PCR (e-PCR) refers to the process of recovering these unique sites in DNA sequences by searching for subsequences that closely match the PCR primers and have the correct order, orientation, and spacing that they could plausibly prime the amplification of a PCR product of the correct molecular weight. A software tool was developed to provide an efficient implementation of this search strategy and allow the sort of en masse searching that is required for modern genome analysis. Some sample searches were performed to demonstrate a number of factors that can affect the likelihood of obtaining a match. Analysis of one large sequence database record revealed the presence of several microsatellite and gene-based markers and allowed the exact base-pair distances among them to be calculated. This example provides a demonstration of how e-PCR can be used to integrate the growing body of genomic sequence data with existing maps, reveal relationships among markers that existed previously on different maps, and correlate genetic distances with physical distances.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.