ArticleThe Biochemical journal1997
Interaction of Nck-associated protein 1 with activated GTP-binding protein Rac.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
- Disulfidptosis mechanisms and therapeutic implications in cancer metabolic reprogramming and future perspectives.Discover oncology · 2025Review
- Identification of sulfur metabolism-related gene signature in osteoarthritis and TM9SF2's sustenance effect on M2 macrophages' phagocytic activity.Journal of orthopaedic surgery and research · 2024Article
- NCK-associated protein 1 regulates metastasis and is a novel prognostic marker for colorectal cancer.Cell death discovery · 2023Article
- Review
- NCKAP1 improves patient outcome and inhibits cell growth by enhancing Rb1/p53 activation in hepatocellular carcinoma.Cell death & disease · 2019Article
- An excitable signal integrator couples to an idling cytoskeletal oscillator to drive cell migration.Nature cell biology · 2013Article
- Under lock and key: spatiotemporal regulation of WASP family proteins coordinates separate dynamic cellular processes.Seminars in cell & developmental biology · 2013Review
- The adaptor molecule Nck localizes the WAVE complex to promote actin polymerization during CEACAM3-mediated phagocytosis of bacteria.PloS one · 2012Article
- Identifying transient protein-protein interactions in EphB2 signaling by blue native PAGE and mass spectrometry.Proteomics · 2011Article
- The Hem protein mediates neuronal migration by inhibiting WAVE degradation and functions opposite of Abelson tyrosine kinase.Developmental biology · 2011Article
- Article
- HSPC300 and its role in neuronal connectivity.Neural development · 2007Article
- Article
- Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis.PLoS biology · 2006Article
- Inclusion of Scar/WAVE3 in a similar complex to Scar/WAVE1 and 2.BMC cell biology · 2005Article
- Dissecting Nck/Dock signaling pathways in Drosophila visual system.International journal of biological sciences · 2005Review
- Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia formation.The EMBO journal · 2004Article
- The GEX-2 and GEX-3 proteins are required for tissue morphogenesis and cell migrations in C. elegans.Genes & development · 2002Article
- Review
- The Drosophila HEM-2/NAP1 homolog KETTE controls axonal pathfinding and cytoskeletal organization.Genes & development · 2000Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterially expressed glutathione S-transferase fusion proteins containing Rac1 were used to identify binding proteins of this Rho family GTPase present in a bovine brain extract. Five proteins of 85, 110, 125, 140 and 170 kDa were detected, all of which were associated exclusively with guanosine 5'-[gamma-thio]triphosphate-bound Rac1, not with GDP-bound Rac1. The 85 and 110 kDa proteins were identified as the regulatory and catalytic subunits respectively of phosphatidylinositol 3-kinase. Several lines of evidence suggested that the 125 kDa protein is identical with Nck-associated protein 1 (Nap1). The mobilities of the 125 kDa protein and Nap1 on SDS/PAGE were indistinguishable, and the 125 kDa protein was depleted from brain extract by preincubation with the Src homology 3 domain of Nck to which Nap1 binds. Furthermore, antibodies to Nap1 reacted with the 125 kDa protein. Nap1 was co-immunoprecipitated with a constitutively active form of Rac expressed in Chinese hamster ovary cells. The observation that complex formation between activated Rac and PAK, but not that between Rac and Nap1, could be reproduced in vitro with recombinant proteins indicates that the interaction of Nap1 with Rac is indirect. The 140 kDa Rac-binding protein is a potential candidate for a link that connects Nap1 to Rac. The multimolecular complex comprising Rac, Nap1 and probably the 140 kDa protein might mediate some of the biological effects transmitted by the multipotent GTPase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.