ArticleProceedings of the National Academy of Sciences of the United States of America1997
Microtubule release from the centrosome.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
95 citing papers in PubMed.
- Patronin promotes the formation of dynamic microtubule seeds in vivo.Journal of cell science · 2026Article
- Fluorescent organelle markers in Cryptococcus neoformans: a versatile toolkit for live-cell subcellular localization.Genetics · 2026Article
- NuSAP Safeguards Centriole Integrity to Mediate CEP57-CEP152 Torus Recruitment for Proper Engagement.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Fluorescent organelle markers inbioRxiv : the preprint server for biology · 2026Article
- Cytoskeletal remodeling via CAMSAP3 downregulation drives resistance to osimertinib in NSCLC cells.Cell death & disease · 2025Article
- Mechanisms of cortical microtubule organization in epidermal keratinocytes.Cellular and molecular life sciences : CMLS · 2025Article
- Microtubule nucleation and γTuRC centrosome localization in interphase cells require ch-TOG.Nature communications · 2023Article
- Combinatorial and antagonistic effects of tubulin glutamylation and glycylation on katanin microtubule severing.Developmental cell · 2022Article
- A cryo-ET survey of microtubules and intracellular compartments in mammalian axons.The Journal of cell biology · 2022Article
- Review
- Microtubule Anchoring: Attaching Dynamic Polymers to Cellular Structures.Frontiers in cell and developmental biology · 2022Review
- Persistent growth of microtubules at low density.Molecular biology of the cell · 2021Article
- Collective effects of XMAP215, EB1, CLASP2, and MCAK lead to robust microtubule treadmilling.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Microtubule-severing enzymes: From cellular functions to molecular mechanism.The Journal of cell biology · 2018Review
- Binucleate germ cells in Caenorhabditis elegans are removed by physiological apoptosis.PLoS genetics · 2018Article
- Non-centrosomal MTs play a crucial role in organization of MT array in interphase fibroblasts.AIMS genetics · 2018Article
- The nano-architecture of the axonal cytoskeleton.Nature reviews. Neuroscience · 2017Review
- Microtubule organization, dynamics and functions in differentiated cells.Development (Cambridge, England) · 2017Review
- A minimal actomyosin-based model predicts the dynamics of filopodia on neuronal dendrites.Molecular biology of the cell · 2017Article
- Article
35 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Although microtubules (MTs) are generally thought to originate at the centrosome, a number of cell types have significant populations of MTs with no apparent centrosomal connection. The origin of these noncentrosomal MTs has been unclear. We applied kinetic analysis of MT formation in vivo to establish their mode of origin. Time-lapse fluorescence microscopy demonstrated that noncentrosomal MTs in cultured epithelial cells arise primarily by constitutive nucleation at, and release from, the centrosome. After release, MTs moved away from the centrosome and tended to depolymerize. Laser-marking experiments demonstrated that released MTs moved individually with their plus ends leading, suggesting that they were transported by minus end-directed motors. Released MTs were dynamic. The laser marking experiments demonstrated that plus ends of released MTs grew, paused, or shortened while the minus ends were stable or shortened. Microtubule release may serve two kinds of cellular function. Release and transport could generate the noncentrosomal MT arrays observed in epithelial cells, neurons, and other asymmetric, differentiated cells. Release would also contribute to polymer turnover by exposing MT minus ends, thereby providing additional sites for loss of subunits. The noncentrosomal population of MTs may reflect a steady-state of centrosomal nucleation, release, and dynamics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.