ArticleProceedings of the National Academy of Sciences of the United States of America1997
Involvement of caspase-dependent activation of cytosolic phospholipase A2 in tumor necrosis factor-induced apoptosis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 209 citations in OpenAlex.
- RhoA/Rho Kinase Mediates Neuronal Death Through Regulating cPLAMolecular neurobiology · 2017Article
- Oligomeric amyloid-beta induces MAPK-mediated activation of brain cytosolic and calcium-independent phospholipase AActa neuropathologica communications · 2017Article
- The tumour suppressor Ras-association domain family protein 1A (RASSF1A) regulates TNF-α signalling in cardiomyocytes.Cardiovascular research · 2014Article
- Cytosolic phospholipase A(2)α protects against Fas- but not LPS-induced liver injury.Journal of hepatology · 2011Article
- cPLA(2)α gene activation by IL-1β is dependent on an upstream kinase pathway, enzymatic activation and downstream 15-lipoxygenase activity: a positive feedback loop.Cellular signalling · 2011Article
- Phospholipase A(2) of peroxiredoxin 6 has a critical role in tumor necrosis factor-induced apoptosis.Cell death and differentiation · 2011Article
- TNF-α induced apoptosis is accompanied with rapid CD30 and slower CD45 shedding from K-562 cells.The Journal of membrane biology · 2011Article
- Role of platelet-activating factor in cell death signaling in the cornea: A review.Molecular neurobiology · 2010Review
- Annexin-II, DNA, and histones serve as factor H ligands on the surface of apoptotic cells.The Journal of biological chemistry · 2010Article
- Lipid mediators in innate immunity against tuberculosis: opposing roles of PGE2 and LXA4 in the induction of macrophage death.The Journal of experimental medicine · 2008Article
- The role and characterization of phospholipase A1 in mediating lysophosphatidylcholine synthesis in Trypanosoma brucei.The Biochemical journal · 2007Article
- Calcium-independent phospholipase A2 localizes in and protects mitochondria during apoptotic induction by staurosporine.The Journal of biological chemistry · 2006Article
- PLA2 activity is required for nuclear shrinkage in caspase-independent cell death.The Journal of cell biology · 2003Article
- Distinct regulation of cytosolic phospholipase A2 phosphorylation, translocation, proteolysis and activation by tumour necrosis factor-receptor subtypes.The Biochemical journal · 2003Article
- Article
- I-PLA(2) activation during apoptosis promotes the exposure of membrane lysophosphatidylcholine leading to binding by natural immunoglobulin M antibodies and complement activation.The Journal of experimental medicine · 2002Article
- Inhibition of mitochondrial calcium-independent phospholipase A2 (iPLA2) attenuates mitochondrial phospholipid loss and is cardioprotective.The Biochemical journal · 2002Article
- TNF ligands and receptors--a matter of life and death.British journal of pharmacology · 2002Review
- Inhibition of TRAIL-induced apoptosis and forced internalization of TRAIL receptor 1 by adenovirus proteins.Journal of virology · 2001Article
- PLIP, a novel splice variant of Tip60, interacts with group IV cytosolic phospholipase A(2), induces apoptosis, and potentiates prostaglandin production.Molecular and cellular biology · 2001Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tumor necrosis factor (TNF)-induced apoptosis is mediated by caspases, which are cysteine proteases related to interleukin 1beta-converting enzyme. We report here that TNF-induced activation of caspases results in the cleavage and activation of cytosolic phospholipase A2 (cPLA2) and that activated cPLA2 contributes to apoptosis. Inhibition of caspases by expression of a cowpox virus-derived inhibitor, CrmA, or by a specific tetrapeptide inhibitor of CPP32/caspase-3, acetyl-Asp-Glu-Val-Asp-aldehyde (Ac-DEVD-CHO), inhibited TNF-induced activation of cPLA2 and apoptosis. TNF-induced activation of cPLA2 was accompanied by a cleavage of the 100-kDa cPLA2 to a 70-kDa proteolytic fragment. This cleavage was inhibited by Ac-DEVD-CHO in a similar manner as that of poly(ADP)ribose polymerase, a known substrate of CPP32/caspase-3. Interestingly, specific inhibition of cPLA2 enzyme activity by arachidonyl trifluoromethylketone (AACOCF3) partially inhibited TNF-induced apoptosis without inhibition of caspase activity. Thus, our results suggest a novel caspase-dependent activation pathway for cPLA2 during apoptosis and identify cPLA2 as a mediator of TNF-induced cell death acting downstream of caspases.
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