ArticleProceedings of the National Academy of Sciences of the United States of America1997
Expression, stability, and membrane integration of truncation mutants of bovine rhodopsin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 40 citations in OpenAlex.
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- Di-acidic motifs in the membrane-distal C termini modulate the transport of angiotensin II receptors from the endoplasmic reticulum to the cell surface.The Journal of biological chemistry · 2011Article
- Anterograde trafficking of nascent α(2B)-adrenergic receptor: structural basis, roles of small GTPases.Current topics in membranes · 2011Review
- Signal peptide peptidase (SPP) assembles with substrates and misfolded membrane proteins into distinct oligomeric complexes.The Biochemical journal · 2010Article
- Anterograde trafficking of G protein-coupled receptors: function of the C-terminal F(X)6LL motif in export from the endoplasmic reticulum.Molecular pharmacology · 2009Article
- Regulation of G protein-coupled receptor export trafficking.Biochimica et biophysica acta · 2007Review
- Evidence that the cysteine-rich domain of Drosophila Frizzled family receptors is dispensable for transducing Wingless.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Different transmembrane domains associate with distinct endoplasmic reticulum components during membrane integration of a polytopic protein.Molecular biology of the cell · 2002Article
- Structure and function in rhodopsin: destabilization of rhodopsin by the binding of an antibody at the N-terminal segment provides support for involvement of the latter in an intradiscal tertiary structure.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- The seven-transmembrane spanning topography of the Alzheimer disease-related presenilin proteins in the plasma membranes of cultured cells.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Cell surface expression of the Alzheimer disease-related presenilin proteins.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Premature termination of protein synthesis by nonsense mutations is at the molecular origin of a number of inherited disorders in the family of G protein-coupled seven-helix receptor proteins. To understand how such truncated polypeptides are processed by the cell, we have carried out COS-1 cell expression studies of mutants of bovine rhodopsin truncated at the first 1, 1.5, 2, 3, or 5 transmembrane segments (TMS) of the seven present in wild-type opsin. Our experiments show that successful completion of different stages in the cellular processing of the protein [membrane insertion, N-linked glycosylation, stability to proteolytic degradation, and transport from the endoplasmic reticulum (ER) membrane] requires progressively longer lengths of the polypeptide chain. Thus, none of the truncations affected the ability of the polypeptides to be integral membrane proteins. C-terminal truncations that generated polypeptides with fewer than two TMS resulted in misorientation and prevented glycosylation at the N terminus, whereas truncations that generated polypeptides with fewer than five TMS greatly destabilized the protein. However, all of the truncations prevented exit of the polypeptide from the ER. We conclude that during the biogenesis of rhodopsin, proper integration into the ER membrane occurs only after the synthesis of at least two TMS is completed. Synthesis of the next three TMS confers a gradual increase in stability, whereas the presence of more than five TMS is necessary for exit from the ER.
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