ArticleApplied and environmental microbiology1997
Influence of cosubstrate concentration on xylose conversion by recombinant, XYL1-expressing Saccharomyces cerevisiae: a comparison of different sugars and ethanol as cosubstrates.
Article in Applied and environmental microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 95 citations in OpenAlex.
- Metabolic engineering for sustainable xylitol production from diverse carbon sources in Pichia pastoris.Microbial cell factories · 2025Article
- Biosynthesis of value-added bioproducts from hemicellulose of biomass through microbial metabolic engineering.Metabolic engineering communications · 2022Review
- Kinetic Modeling ofMetabolites · 2022Review
- Review of Second Generation Bioethanol Production from Residual Biomass.Food technology and biotechnology · 2018Review
- Xylitol production by genetically modified industrial strain of Saccharomyces cerevisiae using glycerol as co-substrate.Journal of industrial microbiology & biotechnology · 2017Article
- Overexpression of NADH-dependent fumarate reductase improves D-xylose fermentation in recombinant Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2013Article
- Simultaneous saccharification and co-fermentation for bioethanol production using corncobs at lab, PDU and demo scales.Biotechnology for biofuels · 2013Article
- Article
- Separate hydrolysis and co-fermentation for improved xylose utilization in integrated ethanol production from wheat meal and wheat straw.Biotechnology for biofuels · 2012Article
- Improving L-arabinose utilization of pentose fermenting Saccharomyces cerevisiae cells by heterologous expression of L-arabinose transporting sugar transporters.Biotechnology for biofuels · 2011Article
- A mutated xylose reductase increases bioethanol production more than a glucose/xylose facilitator in simultaneous fermentation and co-fermentation of wheat straw.AMB Express · 2011Article
- Microbial and bioconversion production of D-xylitol and its detection and application.International journal of biological sciences · 2010Review
- Simultaneous consumption of pentose and hexose sugars: an optimal microbial phenotype for efficient fermentation of lignocellulosic biomass.Applied microbiology and biotechnology · 2010Review
- Cross-reactions between engineered xylose and galactose pathways in recombinant Saccharomyces cerevisiae.Biotechnology for biofuels · 2010Article
- Improving simultaneous saccharification and co-fermentation of pretreated wheat straw using both enzyme and substrate feeding.Biotechnology for biofuels · 2010Article
- Fermentation of mixed glucose-xylose substrates by engineered strains of Saccharomyces cerevisiae: role of the coenzyme specificity of xylose reductase, and effect of glucose on xylose utilization.Microbial cell factories · 2010Article
- Prefermentation improves xylose utilization in simultaneous saccharification and co-fermentation of pretreated spruce.Biotechnology for biofuels · 2009Article
- A short review on SSF - an interesting process option for ethanol production from lignocellulosic feedstocks.Biotechnology for biofuels · 2008Article
- Deletion of the GRE3 aldose reductase gene and its influence on xylose metabolism in recombinant strains of Saccharomyces cerevisiae expressing the xylA and XKS1 genes.Applied and environmental microbiology · 2001Article
- Xylulokinase overexpression in two strains of Saccharomyces cerevisiae also expressing xylose reductase and xylitol dehydrogenase and its effect on fermentation of xylose and lignocellulosic hydrolysate.Applied and environmental microbiology · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conversion of xylose to xylitol by recombinant Saccharomyces cerevisiae expressing the XYL1 gene, encoding xylose reductase, was investigated by using different cosubstrates as generators of reduced cofactors. The effect of a pulse addition of the cosubstrate on xylose conversion in cosubstrate-limited fed-batch cultivation was studied. Glucose, mannose, and fructose, which are transported with high affinity by the same transport system as is xylose, inhibited xylose conversion by 99, 77, and 78%, respectively, reflecting competitive inhibition of xylose transport. Pulse addition of maltose, which is transported by a specific transport system, did not inhibit xylose conversion. Pulse addition of galactose, which is also transported by a specific transporter, inhibited xylose conversion by 51%, in accordance with noncompetitive inhibition between the galactose and glucose/ xylose transport systems. Pulse addition of ethanol inhibited xylose conversion by 15%, explained by inhibition of xylose transport through interference with the hydrophobic regions of the cell membrane. The xylitol yields on the different cosubstrates varied widely. Galactose gave the highest xylitol yield, 5.6 times higher than that for glucose. The difference in redox metabolism of glucose and galactose was suggested to enhance the availability of reduced cofactors for xylose reduction with galactose. The differences in xylitol yield observed between some of the other sugars may also reflect differences in redox metabolism. With all cosubstrates, the xylitol yield was higher under cosubstrate limitation than with cosubstrate excess.
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