ArticleApplied and environmental microbiology1997
Comparison of ribotyping and randomly amplified polymorphic DNA PCR for characterization of Vibrio vulnificus.
Article in Applied and environmental microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 54 citations in OpenAlex.
- Isolation, detection of virulence genes, antibiotic resistance genes, plasmid profile, and molecular typing amongVeterinary world · 2019Article
- Genome-wide SNP-genotyping array to study the evolution of the human pathogen Vibrio vulnificus biotype 3.PloS one · 2014Article
- DNA fingerprinting of Flavobacterium columnare using RAPD-PCR.Indian journal of microbiology · 2010Article
- Evaluation of genotypic and phenotypic methods to distinguish clinical from environmental Vibrio vulnificus strains.Applied and environmental microbiology · 2009Article
- Comparison of direct genome restriction enzyme analysis and pulsed-field gel electrophoresis for typing of Vibrio vulnificus and their correspondence with multilocus sequence typing data.Applied and environmental microbiology · 2007Article
- Emergence of a virulent clade of Vibrio vulnificus and correlation with the presence of a 33-kilobase genomic island.Applied and environmental microbiology · 2007Article
- Pulsed-field gel electrophoresis analysis of Vibrio vulnificus strains isolated from Taiwan and the United States.Applied and environmental microbiology · 2004Article
- Analysis of Vibrio vulnificus from market oysters and septicemia cases for virulence markers.Applied and environmental microbiology · 2003Article
- Population genetics of Vibrio vulnificus: identification of two divisions and a distinct eel-pathogenic clone.Applied and environmental microbiology · 2003Article
- Sequence polymorphism of the 16S rRNA gene of Vibrio vulnificus is a possible indicator of strain virulence.Journal of clinical microbiology · 2003Article
- Random amplified polymorphic DNA analysis to differentiate strains of Vibrio vulnificus isolated from cockles and shrimps.The Malaysian journal of medical sciences : MJMS · 2000Article
- Homogeneity of Danish environmental and clinical isolates of Shewanella algae.Applied and environmental microbiology · 2000Article
- Differentiation of Vibrio alginolyticus strains isolated from Sardinian waters by ribotyping and a new rapid PCR fingerprinting method.Applied and environmental microbiology · 1999Article
- Randomly amplified polymorphic DNA analysis of clinical and environmental isolates of Vibrio vulnificus and other vibrio species.Applied and environmental microbiology · 1999Article
- Effects of salinity and temperature on long-term survival of the eel pathogen Vibrio vulnificus biotype 2 (serovar E).Applied and environmental microbiology · 1999Article
- Heterogeneity among isolates of Vibrio vulnificus recovered from eels (Anguilla anguilla) in Denmark.Applied and environmental microbiology · 1998Article
- Genetic relatedness among environmental, clinical, and diseased-eel Vibrio vulnificus isolates from different geographic regions by ribotyping and randomly amplified polymorphic DNA PCR.Applied and environmental microbiology · 1998Article
- Occurrence of Vibrio vulnificus biotypes in Danish marine environments.Applied and environmental microbiology · 1998Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A total of 85 isolates of Vibrio vulnificus were characterized by ribotyping with a probe complementary to 16S and 23S rRNA of Escherichia coli and by randomly amplified polymorphic DNA-PCR (RAPD-PCR) with a 10-mer oligonucleotide primer. The RAPD-PCR results were scanned, and the images were analyzed with a computer program. Ribotype membranes were evaluated visually. Both the ribotyping and the RAPD-PCR results showed that the collection of strains was genetically very heterogeneous. Ribotyping enabled us to differentiate U.S. and Danish strains and V. vulnificus biotypes 1 and 2, while the RAPD-PCR technique was not able to correlate isolates with sources or to differentiate the two biotypes, suggesting that ribotyping is useful for typing V. vulnificus strains whereas RAPD-PCR profiles may subdivide ribotypes. Two Danish clinical biotype 2 strains isolated from fishermen who contracted the infection cleaning eels belonged to the same ribotype as three eel strains (biotype 2), providing further evidence that V. vulnificus biotype 2 is an opportunistic pathogen for humans. One isolate (biotype 2) from Danish coastal waters also showed the same ribotype as the eel strains. This is, to our knowledge, the first time the isolation of V. vulnificus biotype 2 from coastal waters has been described.
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