ArticleApplied and environmental microbiology1997
ord1, an oxidoreductase gene responsible for conversion of O-methylsterigmatocystin to aflatoxin in Aspergillus flavus.
Article in Applied and environmental microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 81 citations in OpenAlex.
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- Multi-stage nuclear transcriptomic insights of morphogenesis and biparental role changes in Lentinula edodes.Applied microbiology and biotechnology · 2023Article
- Review
- Oxidative Cyclization in Natural Product Biosynthesis.Chemical reviews · 2017Review
- Biosynthesis of oxygen and nitrogen-containing heterocycles in polyketides.Beilstein journal of organic chemistry · 2016Review
- Aflatoxin and deconstruction of type I, iterative polyketide synthase function.Natural product reports · 2014Review
- Current understanding on aflatoxin biosynthesis and future perspective in reducing aflatoxin contamination.Toxins · 2012Review
- Recent advancements in the biosynthetic mechanisms for polyketide-derived mycotoxins.Biopolymers · 2010Review
- Review
- Aflatoxin biosynthesis cluster gene cypA is required for G aflatoxin formation.Applied and environmental microbiology · 2004Article
- Clustered pathway genes in aflatoxin biosynthesis.Applied and environmental microbiology · 2004Review
- Cloning and functional expression of an esterase gene in Aspergillus parasitcus.Mycopathologia · 2002Article
- Life history and developmental processes in the basidiomycete Coprinus cinereus.Microbiology and molecular biology reviews : MMBR · 2000Review
- Requirement of monooxygenase-mediated steps for sterigmatocystin biosynthesis by Aspergillus nidulans.Applied and environmental microbiology · 2000Article
- Enzymatic formation of G-group aflatoxins and biosynthetic relationship between G- and B-group aflatoxins.Applied and environmental microbiology · 1999Article
- Characterization of the critical amino acids of an Aspergillus parasiticus cytochrome P-450 monooxygenase encoded by ordA that is involved in the biosynthesis of aflatoxins B1, G1, B2, and G2.Applied and environmental microbiology · 1998Article
- Overexpression of aflR Leads to Upregulation of Pathway Gene Transcription and Increased Aflatoxin Production in Aspergillus flavus.Applied and environmental microbiology · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Among the enzymatic steps in the aflatoxin biosynthetic pathway, the conversion of O-methylsterigmatocystin to aflatoxin has been proposed to be catalyzed by an oxidoreductase. Transformants of Aspergillus flavus 649WAF2 containing a 3.3-kb genomic DNA fragment and the aflatoxin biosynthesis regulatory gene aflR converted exogenously supplied O-methylsterigmatocystin to aflatoxin B1. A gene, ord1, corresponding to a transcript of about 2 kb was identified within the 3.3-kb DNA fragment. The promoter region presented a putative AFLR binding site and a TATA sequence. The nucleotide sequence of the gene revealed an open reading frame encoding a protein of 528 amino acids with a deduced molecular mass of 60.2 kDa. The gene contained six introns and seven exons. Heterologous expression of the ord1 open reading frame under the transcriptional control of the Saccharomyces cerevisiae galactose-inducible gal1 promoter results in the ability to convert O-methylsterigmatocystin to aflatoxin B1. The data indicate that ord1 is sufficient to accomplish the last step of the aflatoxin biosynthetic pathway. A search of various databases for similarity indicated that ord1 encodes a cytochrome P-450-type monooxygenase, and the gene has been assigned to a new P-450 gene family named CYP64.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.