ArticleJournal of bacteriology1997
Characterization of a glucose-repressed pyruvate kinase (Pyk2p) in Saccharomyces cerevisiae that is catalytically insensitive to fructose-1,6-bisphosphate.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
53 citing papers in PubMed, 120 citations in OpenAlex.
- Article
- Article
- Reversible amyloids of pyruvate kinase couple cell metabolism and stress granule disassembly.Nature cell biology · 2021Article
- Increasing glycolysis by deletion of kcs1 and arg82 improved S-adenosyl-L-methionine production in Saccharomyces cerevisiae.AMB Express · 2021Article
- Gene expression regulates metabolite homeostasis during the Crabtree effect: Implications for the adaptation and evolution of Metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Impact of the Whole Genome Duplication Event on PYK Activity and Effects of a PYK1 Mutation on Metabolism inFrontiers in molecular biosciences · 2021Article
- Pyruvate kinase variant of fission yeast tunes carbon metabolism, cell regulation, growth and stress resistance.Molecular systems biology · 2020Article
- Molecular evolutionary engineering of xylose isomerase to improve its catalytic activity and performance of micro-aerobic glucose/xylose co-fermentation inBiotechnology for biofuels · 2019Article
- Regulation of Cellular Metabolism through Phase Separation of Enzymes.Biomolecules · 2018Review
- Article
- l-Arabinose triggers its own uptake via induction of the arabinose-specific Gal2p transporter in an industrialBiotechnology for biofuels · 2018Article
- Glycolytic Functions Are Conserved in the Genome of the Wine Yeast Hanseniaspora uvarum, and Pyruvate Kinase Limits Its Capacity for Alcoholic Fermentation.Applied and environmental microbiology · 2017Article
- Effect of the cancer specific shorter form of human 6-phosphofructo-1-kinase on the metabolism of the yeast Saccharomyces cerevisiae.BMC biotechnology · 2017Article
- Increased rates of protein evolution and asymmetric deceleration after the whole-genome duplication in yeasts.BMC evolutionary biology · 2017Article
- Regulation of SESAME-mediated H3T11 phosphorylation by glycolytic enzymes and metabolites.PloS one · 2017Article
- A novel pyruvate kinase and its application in lactic acid production under oxygen deprivation in Corynebacterium glutamicum.BMC biotechnology · 2016Article
- Redox-dependent Regulation of Gluconeogenesis by a Novel Mechanism Mediated by a Peroxidatic Cysteine of Peroxiredoxin.Scientific reports · 2016Article
- Redirection of pyruvate flux toward desired metabolic pathways through substrate channeling between pyruvate kinase and pyruvate-converting enzymes in Saccharomyces cerevisiae.Scientific reports · 2016Article
- Flux Control in Glycolysis Varies Across the Tree of Life.Journal of molecular evolution · 2016Article
- Screening the yeast genome for energetic metabolism pathways involved in a phenotypic response to the anti-cancer agent 3-bromopyruvate.Oncotarget · 2016Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have characterized the gene YOR347c of Saccharomyces cerevisiae and shown that it encodes a second functional pyruvate kinase isoenzyme, Pyk2p. Overexpression of the YOR347c/PYK2 gene on a multicopy vector restored growth on glucose of a yeast pyruvate kinase 1 (pyk1) mutant strain and could completely substitute for the PYK1-encoded enzymatic activity. PYK2 gene expression is subject to glucose repression. A pyk2 deletion mutant had no obvious growth phenotypes under various conditions, but the growth defects of a pyk1 pyk2 double-deletion strain were even more pronounced than those of a pyk1 single-mutation strain. Pyk2p is active without fructose-1,6-bisphosphate. However, overexpression of PYK2 during growth on ethanol did not cause any of the deleterious effects expected from a futile cycling between pyruvate and phosphoenolpyruvate. The results indicate that the PYK2-encoded pyruvate kinase may be used under conditions of very low glycolytic flux.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.