ArticleThe Journal of membrane biology1996
The SpTRK gene encodes a potassium-specific transport protein TKHp in Schizosaccharomyces pombe.
Article in The Journal of membrane biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 41 citations in OpenAlex.
- Exomer Is Part of a Hub Where Polarized Secretion and Ionic Stress Connect.Frontiers in microbiology · 2021Article
- Potassium and sodium transport in non-animal cells: the Trk/Ktr/HKT transporter family.Cellular and molecular life sciences : CMLS · 2010Review
- Diversity in expression patterns and functional properties in the rice HKT transporter family.Plant physiology · 2009Article
- Response of fission yeast to toxic cations involves cooperative action of the stress-activated protein kinase Spc1/Sty1 and the Hal4 protein kinase.Molecular and cellular biology · 2005Article
- Trk1 and Trk2 define the major K(+) transport system in fission yeast.Journal of bacteriology · 2000Article
- Evolutionary relationship between K(+) channels and symporters.Biophysical journal · 1999Article
- Structural models of the KtrB, TrkH, and Trk1,2 symporters based on the structure of the KcsA K(+) channel.Biophysical journal · 1999Article
- 16th SMYTE (Small Meeting on Yeast Transport and Energetics). Casta-Papiernicka, Slovakia, September 23-27, 1998. Abstracts.Folia microbiologica · 1999Article
- Divalent cation block of inward currents and low-affinity K+ uptake in Saccharomyces cerevisiae.Journal of bacteriology · 1999Article
- K(+)-fluxes through plasma membrane of Schizosaccharomyces pombe as measured by whole-cell patch clamp.Folia microbiologica · 1998Article
- The spTRK gene encodes a potassium-specific transport protein TKHp in Schizosaccharomyces pombe.The Journal of membrane biology · 1997Article
- Potassium transport in Schizosaccharomyces pombe.Folia microbiologica · 1997Article
- Functional characterization of Schizosaccharomyces pombe genes cloned in potassium transport defective yeast strains.Folia microbiologica · 1996Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Complementary DNAs involved in potassium transport in Schizosaccharomyces pombe were selected by complementation of defective K+ uptake in a trk1 trk2 mutant of Saccharomyces cerevisiae. Here we describe the SpTRK gene that encodes a protein of 833 amino acids. The predicted structure contains 12 putative membrane-spanning domains and resembles various high- and low-affinity systems for K+ transport in yeasts and plants. TKHp, the product of SpTRK exhibits high homology to TRK1 and TRK2 of Saccharomyces cerevisiae as well as to HKT1 of Triticum aestivum, but is not related to HAK1 of another ascomycete, Schwanniomyces occidentalis, suggesting that different routes for potassium uptake evolved independently. This protein is a potassium-specific transporter since functional analysis of the SpTRK complemented mutant strain of Sacch. cerevisiae revealed potassium transport affinities and uptake characteristics similar to those obtained in wild-type Sch. pombe. Patch-clamp analysis in the whole-cell mode confirmed the TKHp-mediated inward current in the complemented strain. The inward current increased by acidification of the extracellular medium thereby suggesting a mechanism of K+H+ cotransport. The inward current is not detectable when external K+ is substituted by Na+, documenting a distinct cation specificity of the protein.
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